九种降糖药物的高效液相色谱分析Analysis of Nine Antidiabetic Drugs by HPLC
兰玉坤,龚士学
LAN Yu-kun, GONG Shi-xue (Chongqing Institute for Drug Control
摘要(Abstract)：
目的建立一种能够同时分离九种降糖药物的HPLC方法。方法Waters Symmetry C18色谱柱,流动相为甲醇-0.1%乙酸铵(用冰醋酸调pH值为5.0)(70︰30)流速为1.0ml/min,检测波长为233nm,进样量为10μl。结果九种物质的分离度都达到1.9以上,重复进样5次,保留时间的RSD都小于0.1%,峰面积的RSD都小于1.68%,回收率较好。结论本方法简单,重复性好,可同时分离9种降糖药物。
Objective To establish an HPLC method for separating nine antidiabetic drugs. Methods Chromatography was performed on Waters Symmetry C18 column (150 mm×4.6 mm, 5 μm), with a mobile phase of methanol and 0.1% ammonium acetate (adjusted to pH=5.0 with glacial acetic acid) (70~30). The flow rate was 1.0 ml/min, the detection wavelength was at 233 nm, and the sample size was 10 μl. Results All the 9 kinds of drug exhibited good resolution (r>1.9). RSD of the retention time < 0.1%, RSD of the peak areas <1.68% (n=5). Conclusion This method is simple and has good repeatability.
关键词(KeyWords)：
降糖药物;高效液相色谱;DAD检测
antidiabetic drugs; HPLC; DAD detection
基金项目(Foundation): 重庆市自然科学基金资助项目(编号:CSTC2007BB5254)
作者(Author):
兰玉坤,龚士学
LAN Yu-kun, GONG Shi-xue (Chongqing Institute for Drug Control
参考文献(References)：
- [1]郭彩华,蔡慧农,卢珍华.糖尿病的生化机理浅释[J].生物学通报.2003,38(3):24-25.
- [2]陈睿杰.糖尿病治疗药物的研究现状[J],广东药学院学报.2001,17(2):131-133.
- [3]张翠欣,王中孝.降糖药的研究进展[J].中国药学杂志.2002,37(5):328-330.
- [4]钱亚萍.高效液相色谱法测定消渴灵片中格列苯脲含量[J].中国药业.2007,16(1):16-17.
- [5]夏顺宁,王贞媛,李丹.H PLC-M S法鉴别糖乐舒胶囊中非法添加的格列苯脲、格列齐特、格列美脲成分.江苏药学与临床研究.2006,14(6):363-365.
- [6]郭继芬,陈笑艳,钟大放.6种口服降糖药的液相色谱-质谱分析[J].分析测试学报.2000,19(6):5-8.