RRLC-MS/MS同时测定罂粟壳药材中5种生物碱的含量Determination of Five Alkaloids in Pericarpium Papaveris by RRLC-MS/MS
李纯,栗建明,顾利红
LI Chun,LI Jianming,GU Lihong
摘要(Abstract)：
目的建立快速液相色谱-串联质谱法(RRLC-MS/MS)同时测定罂粟壳药材中5种生物碱(可待因、吗啡、罂粟碱、蒂巴因和那可丁)的含量。方法色谱柱为菲罗门Luna C18(150 mm×2.00 mm,3μm),甲醇-10 mmol·L~(-1)乙酸氨水为流动相梯度洗脱,电喷雾离子源正离子模式,选择性反应监测。结果 5种目标成分在各自线性范围内线性关系良好(r>0.997 6),加样回收率在96.1%~99.4%之间,RSD为2.5%~5.0%。5种生物碱总量为0.505%~0.760%,依次为吗啡>那可丁>可待因>罂粟碱>蒂巴因。结论本方法能快速、简便、有效地测定罂粟壳药材中多种指标性成分,为临床应用的质量标准提供参考。
OBJECTIVE To develop a method for the determination of five alkaloids( codein,morphine,papaverine,thebaine and narcotine) in Pericarpium Papaveris using rapid resolution liquid chromatography coupled to triple quadrupole mass spectrometry( RRLC-MS/MS). METHODS The separation was performed on a Phenomenex Luna C18 column( 150 mm×2.0 mm,3 μm),using methanol -10 mmol·L~(-1) ammonium acetate as mobile phase with gradient elution. The five target components were detected in multiplereaction monitoring( MRM) mode by mass spectrometry with electrospray ionization( ESI) source operated in positive ionization mode.RESULTS Good linearity was achieved in their respective linear ranges( r>0.997 6),and the recoveries ranged from 96.1%-98.4%with the RSDs ranged from 2.5%-5.0%. The quantitative results showed that the overall content of five alkaloids was 0.505%-0.760%.The largest content was morphine,with the second narcotine,and smallest thebaine. Codein and papaverine had different size.CONCLUSION The method is rapid,simple and effective for the target components quantification in Pericarpium Papaveris. It is supplied for quality standard in clinic study.
关键词(KeyWords)：
罂粟壳;生物碱;含量测定;快速液相色谱-串联质谱法
Pericarpium Papaveris;alkaloids;content determination;RRLC-MS/MS
基金项目(Foundation):
作者(Author):
李纯,栗建明,顾利红
LI Chun,LI Jianming,GU Lihong
参考文献(References)：
- [1]陈京荔,赵京春.医疗机构中药麻醉药品(罂粟壳)的使用与管理[J].世界中医药,2014,9(10):1370-1372.
- [2]赵万林.罂粟壳中毒2例报告[J].中外医疗,2008,27(29):164.
- [3]贺荣莉,唐甜甜.纳洛酮救治新生儿罂粟壳煎剂中毒一例[J].中国新生儿科杂志,2010,25(4):207.
- [4]陈鸣.中国麻醉药品管理历史沿革及医疗机构麻醉药品管理[J].中国医院药学杂志,2015,35(5):375-379.
- [5]陈鸣.罂粟壳的临床应用与管理现状[J].中国药房,2016,27(25):3461-3463.
- [6]中华人民共和国国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:369-370.
- [7]李雕,李进瞳,曾燕,等.不同等级罂粟壳有效成分含量的研究[J].中国中药杂志,2010,35(17):2246-2249.
- [8]陈辉,陈兵,柏川,等.2-(1H-苯并咪唑-2-基)-3-苯丙烯腈类化合物的电喷雾质谱裂解方式研究[J].广东药学院学报,2016,32(2):169-175.