利用蛋白质转导结构域进行生物大分子的胞内转运Intracellular delivery of bioloyically active macromolecules by protein transduction domains (PTDs)
万敏,吴汝林,刘均洪
Wan Ming ;Wu Rulin;Liu Junhong
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摘要(Abstract)：
由于细胞膜的存在限制了生物利用度,大分子物质的胞内转运还存在许多问题。最近通过对蛋白质转导结构域的研究已经排除了这一障碍。应用蛋白质转导结构域将大分子蛋白质、核酸、病毒、小分子物质转运到目标细胞内已引起了人们越来越多的关注。虽然十年前就发现了蛋白质转导结构域,如触足蛋白、Tat及Vp22,但其作用机制刚刚明确并开始被广泛的应用。除了富含精氨酸的蛋白质转导结构域,我们通过流式细胞光密度的测定和酶的测定,还发现赖氨酸同聚物也能够介导许多细胞的转导过程。蛋白质转导结构域,有天然存在的和人工合成的,已越来越广泛的应用于将生物活性物质转运到细胞内以便更好的治疗某些疾病,如癌症、中风、关节炎等等。
Intracellular delivery of macromolecules remains problematic because of the bioavailability restriction imposed by the cell membrance . Recent studies on protein transduction domains have circumvented this barrier. Increasing attention has been directed towards employing protein transduction domains ( PTDs) to efficiently deliver full length proteins, nucleic acid, viruses and small molecules to cellular targets. Although discovered over a decade ago, the mechanisms by PTDs,such as antennapedia.TAT and Vp22,transduce cells with efficiency are only now beginning to be understood and widely exploited. In addition to the characterized arginine-rich PTDs ,we have demonstrated the lysine homopolymers are able to mediate transduction of a wide variety of cell types as measured by flow cytometic and enzymatic assays. Protein transduction domains (PTDs) , both naturally and synthetic,have been increasing employed to deliver bioloyically active agents to a variety of cell types for improved treatments of some diseases, such as cancer, stroke, arthritis and so on.
关键词(KeyWords)：
蛋白质转导结构域;转运
protein transduction domains; deliver
基金项目(Foundation): 国家自然科学基金资助课题,批准号:20176019
作者(Authors):
万敏,吴汝林,刘均洪
Wan Ming ;Wu Rulin;Liu Junhong
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