高效液相色谱法测定葛根异黄酮中葛根素的含量Determination of puerarin in isoflavones of Pueraria iobata (willd.)Ohwi.by HPLC
任永康
Ren Yongkang(Guangdong Yantang Biochemistry Pharmaceutical Co Ltd;
摘要(Abstract)：
目的 :探讨用高效液相色谱法定量测定葛根素的含量。方法 :色谱柱 大连SpherisorbC18( 10 μm ,4 6mm× 2 50mm) ;流动相 水 (用冰醋酸调至pH3 0 ) 甲醇 ( 80∶2 0 ) ;二极管阵列检测器 ,检测波长 2 50nm。结果 :葛根素与 3' 甲氧基葛根素完全分离。浓度线性范围为 2 0～ 180 μg/ml(r =0 9999) ,平均加样回收率为10 1 7% ,RSD =0 4 3% (n =5)。结论 :本法简便、准确 ,重现性好 ,可用于葛根素的质量控制。
Objective: To investigate a HPLC method for quantitative determining puerarin Method: The chromatographic conditions were as follow: Dalian Spherisorb C 18 (10μm,4 6mm×250mm),water(adjusted to pH=3 0 with glacial acetic acid) methanol (volume ratio 80∶20) as the mobile and a Waters 996 photodiode array detector with wavelengh at 250nm Result:Good separation was achieved between puerarin and 3' methoxy puerarin The calibration curve was linear within 20～180μg·ml -1 and the correlation coefficient was 0 9999 The average recovery rate and RSD were 101 7% and 0 43%(n=5) Conclusion:This method is simple,accurate and reproducible and can be used for the quality control of puerarin
关键词(KeyWords)：
高效液相;色谱法;葛根素;3'-甲氧基葛根素
HPLC,Puerarin,3' methoxy puerarin
基金项目(Foundation):
作者(Authors):
任永康
Ren Yongkang(Guangdong Yantang Biochemistry Pharmaceutical Co Ltd;
参考文献(References)：
- 1宋振玉 中草药现代研究 北京医科大学 -中国协和医科大学联合出版社 ,1995 :2 5 8～ 30 5
- 2李汶 ,黄诺嘉 脑脉康泰胶囊中葛根素的HPLC测定 中成药 1995 ,17(6 ) :10～ 11
- 3项勤 ,阮文兴 ,叶丽华 HPLC法测定养阴降糖片葛根素的含量 现代应用药学 1996 ,13(6 ) :5 8～ 5 9
- 4王栋 ,刘宁 高效液相色谱法测定小儿腹泻宁袋泡剂中葛根素含量的方法研究 药物分析杂志 1997,17(5 ) :345～346
- 5房其年 ,吴鹏 ,杨林 葛根素的高效液相色谱分离和含量测定 药学学报 1983,18(9) :6 95～ 6 99
- 6中国药典 (一部 ) 1995 :附录 37～ 39