杜碧莹
DU Bi-ying (Guangzhou Institute of Drug Control

• 1:广州市药品检验所

摘要(Abstract)：

目的建立高效液相色谱法,改进注射用水溶性维生素中维生素B12的含量测定方法。方法色谱柱为COSMOSIL C18(250mm×4.6mm×5μm),流动相为甲醇-水(25∶75);流速为1.2ml/min;柱温为30℃,检测波长为550nm。结果维生素B12在每毫升含0.25～0.75μg的浓度范围内线性关系良好,r=0.9998(n=5)。平均回收率为99.2%,相对标准偏差值(RSD)为0.8%(n=9)。结论本方法简便、准确,不受其他成分的干扰,可用于测定注射用水溶性维生素中维生素B12的含量。
Objective To establish an HPLC method to improve the content assay method for vitamin B12 in water-soluble vitamins for injection.Methods COSMOSIL C18 (250 mm×4.6 mm,5 μm) was used as the column.The mobile phase was consisted of methanol-water (25∶75).The flow rate was 1.2 ml /min,the column temperature was 30℃,and the detection wavelength was 550 nm.Results Vitamins B12 presented a good linear ship in the range of 0.25～0.75 μg,r=0.9998 (n=5).The average recovery was 99.2%,RSD=0.8% (n=9).Conclusion The present method is simple and accurate for the content determination of vitamin B12 in water-soluble vitamins for injection,and it also has the advantage of preventing the interference from other compounds.

关键词(KeyWords)： 注射用水溶性维生素;维生素B12;含量测定
water-soluble vitamins;vitamin B12;content assay

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作者(Author): 杜碧莹
DU Bi-ying (Guangzhou Institute of Drug Control

参考文献(References)：

• 1国家药典委员会.中国药典(2005年版第二部).北京:化学工业出版社,2005:668.
• 2王虎,苏玉永,郑思维.高效液相色谱法测定复方维生素胶囊中维生素B12的含量.中国药房,2006,17(6):460-461.
• 3刘敏,郑伟涛.HPLC法测定功能性饮料中多种B族维生素的含量.饮料工业,2006,9(1):39-41.
• 4成志强,孙成均,黎源倩.反相高效液相色谱法同时测定食物和多维片中8种水溶性维生素.分析化学研究简报,2001,29(9):1068-1071.