麝香草酚中有机溶剂残留量测定方法的建立Determination of in material Material of Thymolum
梁北梅,骆维康,安穗伟
LIANG Bei-mei,Wei-kang,Sui-wei(Guangzhou Pharmaceutical Industrial Research institute
摘要(Abstract)：
目的建立麝香草酚中有机残留溶剂1,2-二氯乙烷的气相色谱测定法。方法色谱柱为DB-624毛细管柱,FID检测器,进样口温度200℃、检测器温度250℃,载气为氮气。柱温采取程序升温:起始温度为60℃,保持10min,以每分钟50℃的速度升至210℃保持10min。结果线性范围为0.00312～0.0250mg/ml(r=0.9999);方法的检出限(S/N=3)为5.1×10-4μg,进样精密度良好。结论本方法简便、快速、灵敏,可用于麝香草酚残留有机溶剂的测定。
Objective To establish gas chromatography method for determination of organic solvent residue in material of Thymolum.Methods The DB-624 column (30 m×0.322 mm×1.8 μm) used,FID detector,the sample was dissolved in N,N-dimethylformanide,the injector temperature was 200 ℃,the detector temperature was 250 ℃.The carry gas was nitrogen.The programming temperature of column,the initial temperature was kept at 60 ℃ for 10 min,the temperature was raised to 210℃at rate of 50 ℃ /min,and maintain for 10 min.Results 1,2-Dichloroethane was not detected in three batches of Thymolum.The standard curves linear the range 0.00312～0.0250 mg /ml(r=0.99991).The limits of detection were 5.1×10-4 g (S/N=3).Conclusion The method is accurate,simple,and accurate.It can be used to determine the organic solvent residue in material of Thymolum.
关键词(KeyWords)：
气相色谱法;麝香草酚;溶剂残留
gas chromatography;Thymolum;organic solvent residue
基金项目(Foundation):
作者(Author):
梁北梅,骆维康,安穗伟
LIANG Bei-mei,Wei-kang,Sui-wei(Guangzhou Pharmaceutical Industrial Research institute