邹国盛,梅花,潘霞,萧灿宏,张霞,林育纯,刘雅兰,何琳
ZOU Guosheng,MEI Hua,PAN Xia,XIAO Canhong,ZHANG Xia,LIN Yuchun,LIU Yalan,HE Lin

• 1:广东省第二人民医院
• 2:广东药科大学

摘要(Abstract)：

目的建立测定利丙双卡因醇质体中利多卡因和丙胺卡因含量及包封率的高效液相色谱(HPLC)法。方法采用葡聚糖凝胶微柱离心法分离醇质体与游离药物,并通过HPLC法测定醇质体的包封率。采用Hypersil ODS2色谱柱(4.6 mm×150 mm,5μm),以0.05%磷酸二氢铵(p H7.0)-甲醇(30∶70,V/V)为流动相,流速为1.0 m L·min~(-1),检测波长为254 nm。结果利多卡因与丙胺卡因在10.00~100.0μg·m L~(-1)浓度范围内与峰面积呈良好的线性关系,r=0.999 5,回收率在98%~102%范围内(RSD<4%),3批醇质体中利多卡因包封率分别为82.46%,78.99%,80.73%;丙胺卡因包封率分别为84.04%,81.59%,84.13%。结论建立的微柱离心-高效液相色谱法便捷,重现性好,可用于测定利丙双卡因醇质体的包封率。
OBJECTIVE To establish a HPLC method for determination of the content and entrapment efficiency of lidocaine and prilocaine in compound ethosomes. METHODS Glucose gel minicolumn centrifugation was employed to separate the free drug from the ethosomes. The content and entrapment efficiency of lidocaine and prilocaine was determined by HPLC. Chromatographic conditions were as follows: column was Hypersil ODS2( 4.6 mm × 150 mm,5 μm) and the mobile phase was 0. 05% ammonium dihydrogen phosphate( pH= 7.0)-methanol( 30 ∶ 70,V/V) with the flow rate of 1.0 m L·min~(-1),and detection wavelength was 254 nm. RESULTS The calibration curve for lidocaine and prilocaine were linear in the range of 10.00-100.0 μg·m L~(-1)( r= 0.999 5).The average recovery were among 98%-102%( RSD<4%). The entrapment efficiency of lidocaine in three ethosomes was 82.46%,78.99%,80.73% respectively.The entrapment efficiency of prilocaine in three ethosomes was 84. 04%,81. 59%,84. 13% respectively. CONCLUSION The established minicolumn centrifugation-HPLC method is simple,reproducible,and applicable for the determination of entrapment efficiency of lidocaine and prilocaine in compound ethosomes.

关键词(KeyWords)： 利多卡因;丙胺卡因;醇质体;包封率;高效液相色谱法
lidocaine;prilocaine;ethosome;entrapment efficiency;HPLC

Abstract:

Keywords:

基金项目(Foundation): 广东省医院药学研究基金(2016MZ03)

作者(Author): 邹国盛,梅花,潘霞,萧灿宏,张霞,林育纯,刘雅兰,何琳
ZOU Guosheng,MEI Hua,PAN Xia,XIAO Canhong,ZHANG Xia,LIN Yuchun,LIU Yalan,HE Lin

参考文献(References)：

• [1]王荣,王晓娟,顾宜,等.利多卡因/丙胺卡因复方凝胶制备工艺的研究[J].解放军药学学报,2009,25(5):398-400.
• [2]贺亚静,武学鑫,周洁,等.复方利多卡因乳膏体外透皮研究[J].中国药业,2015,24(15):19-21.
• [3]安珂瑶,孙勇.醇质体的研究进展[J].中国药房,2011,22(5):463-465.
• [4]李理宇,王洪权.新型经皮给药载体——醇质体的研究进展[J].中国新药杂志,2010,19(1):33-38.
• [5]王素芳,刘利萍,边可君.微柱凝胶离心-HPLC测定酮康唑醇质体包封率[J].中国现代应用药学,2011,28(6):527-530.
• [6]于燕燕,赵继会,冯年平,等.微柱离心-HPLC法测定鬼臼毒素醇质体的包封率研究[J].中草药,2010,41(10):1634-1637.
• [7]兰树敏,陈燕,卢慧勤,等.HPLC法测定蛇床子素脂质体中药物含量及包封率[J].今日药学,2016,26(4):229-230.
• [8]孙淑娟,谷大建,杨久丽,等.高效液相色谱法测定复方利多卡因乳膏中丙胺卡因与利多卡因的含量[J].中国医院药学杂志,2002,22(11):33-35.
• [9]李富利,朱晓亮,彭学标,等.利多卡因醇质体的组方研究及其包封率测定[J].皮肤性病诊疗学杂志,2011,18(1):26-29.
• [10]纪宏宇,王瑞红,潘卫三,等.米诺地尔醇质体中药物含量及包封率的测定[J].哈尔滨医科大学学报,2016,44(3):224-226.