今日药学

2017, v.27(06) 388-389+403

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波长切换HPLC法同时测定补肾强身胶囊中3种成分
Determination of Icariin,Epimedin C and Specnuezhenide in BushenQiangshen Capsule

邬伟魁;
WU Weikui;Meizhou Institute for Food and Drug Control;

摘要(Abstract):

目的建立同时测定补肾强身胶囊中淫羊藿苷、朝藿定C、特女贞苷的高效液相色谱(HPLC)法。方法采用SHIMADZUVP-ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速1.0 mL·min(-1),柱温35℃,检测波长0(-1),柱温35℃,检测波长030 min为224 nm,3030 min为224 nm,3045 min为270 nm。结果淫羊藿苷、朝藿定C、特女贞苷分别在3.5545 min为270 nm。结果淫羊藿苷、朝藿定C、特女贞苷分别在3.55177.70μg·mL177.70μg·mL(-1)(r=0.999 9)、3.98(-1)(r=0.999 9)、3.98199.07μg·mL199.07μg·mL(-1)(r=0.999 8)、3.49(-1)(r=0.999 8)、3.49174.97μg·mL174.97μg·mL(-1)(r=0.999 9)浓度范围内线性关系良好。结论该方法可用于补肾强身胶囊的质量控制。
OBJECTIVE To establish an HPLC method for simultaneous determination of icariine,epimedin C and specnuezhenide in Bushen Qiangshen Capsule. METHODS The chromatographic separation was carried out on a SHIMADZUVP-ODS column( 250 mm × 4. 6 mm,5 μm) with mobile phase consisted of acetonitrile-water in gradient elution mode. The flow rate was1.0 mL·min(-1)(r=0.999 9)浓度范围内线性关系良好。结论该方法可用于补肾强身胶囊的质量控制。
OBJECTIVE To establish an HPLC method for simultaneous determination of icariine,epimedin C and specnuezhenide in Bushen Qiangshen Capsule. METHODS The chromatographic separation was carried out on a SHIMADZUVP-ODS column( 250 mm × 4. 6 mm,5 μm) with mobile phase consisted of acetonitrile-water in gradient elution mode. The flow rate was1.0 mL·min(-1). The detection wavelength was 224 nm( 0-30 min) and 270 nm( 30-45 min),and the column temperature was 35 ℃.RESULTS The linear range of icariine,epimedin C and specnuezhenide was 3. 55-177. 70 μg·mL(-1). The detection wavelength was 224 nm( 0-30 min) and 270 nm( 30-45 min),and the column temperature was 35 ℃.RESULTS The linear range of icariine,epimedin C and specnuezhenide was 3. 55-177. 70 μg·mL(-1)( r = 0.999 9),3.98-199.07μg·mL(-1)( r = 0.999 9),3.98-199.07μg·mL(-1)( r= 0.999 8),3.49-174.97μg·mL(-1)( r= 0.999 8),3.49-174.97μg·mL(-1)( r= 0.999 9),respectively. CONCLUSION The established standard is applicable for the quality control of Bushen Qiangshen Capsule.

关键词(KeyWords): 补肾强身胶囊;淫羊藿苷;朝藿定C;特女贞苷
Bushen Qiangshen capsules;icariin;epimedin C;specnuezhenide

Abstract:

Keywords:

基金项目(Foundation): 广东省“十三五”规划中药标准提高项目(2016)

作者(Authors): 邬伟魁;
WU Weikui;Meizhou Institute for Food and Drug Control;

参考文献(References):

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