罗丹;陈丹;白曦晨;向极钎;樊建元;刘卫;
LUO Dan;CHEN Dan;BAI Xichen;XIANG Jiqian;FAN Jianyuan;LIU Wei;Wuhan Bestcarrier Biotechnology Co., Ltd.;College of Life Science and Technology, Huazhong University of Science and Technology;Enshi Academy of Agricultural Sciences;Enshi Savant Ecological Agriculture Development Co., Ltd.;National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology;

• 1:武汉百思凯瑞生物科技有限公司
• 2:华中科技大学生命科学与技术学院
• 3:恩施州农业科学院
• 4:恩施思慧益臣生态农业发展有限公司

摘要(Abstract)：

目的研究富硒青钱柳-黄精复方对高尿酸血症模型小鼠降尿酸作用的影响。方法采用氧嗪酸钾建立高尿酸血症小鼠模型,以生理盐水、阳性药别嘌呤醇和富硒青钱柳-黄精复方连续灌胃给药14 d。检测高尿酸血症小鼠血清尿酸(UA)、尿素氮(BUN)及肌酐(CR)水平,测定肝脏黄嘌呤氧化酶(XOD)、黄嘌呤脱氢酶(XDH)和腺苷脱氢酶(ADA)活力,HE染色观察肝脏、肾脏组织病理学变化。结果连续给药14 d后,适宜剂量的富硒青钱柳-黄精复方能显著降低高尿酸血症小鼠的UA、CR及BUN水平,抑制肝脏ADA、XOD及XDH酶活力,并能改善肝脏、肾脏组织的病理损伤程度。结论富硒青钱柳-黄精复方对高尿酸血症小鼠具有显著降尿酸功效,且肝肾不良反应较别嘌呤醇小。
OBJECTIVE To study the effect of the selenium-enriched Cyclocarya paliurus and Polygonatum sibiricum compounds on lowering uric acid in mice with hyperuricemia. METHODS The mice model of hyperuricemia were established with potassium oxazinate, and the hyperuricemia mice were administered with normal saline, positive drug allopurinol, and selenium-enriched Cyclocarya paliurus and Polygonatum sibiricum compounds for 14 days respectively. The serum uric acid(UA), urea nitrogen(BUN) and creatinine(CR) levels in hyperuricemia mice were detected, and liver xanthine oxidase(XOD), xanthine dehydrogenase(XDH) and adenosine dehydrogenase(ADA) vitality were determine. And the histopathological changes of liver and kidney were observed by HE staining. RESULTS After 14 days of continuous administration, the appropriate dose of the selenium-enriched Cyclocarya paliurus and Polygonatum sibiricum compounds reduced the levels of UA, CR and BUN in hyperuricemia mice significantly, inhibited the activity of liver ADA, XOD and XDH enzymes, and improved the degree of pathological damage to the liver and kidney tissue. CONCLUSION It shows that the selenium-enriched Cyclocarya paliurus and Polygonatum sibiricum compounds has significant effect on lowering uric acid in hyperuricemia mice, and the liver and kidney adverse reactions are less than allopurinol.

关键词(KeyWords)： 富硒青钱柳;黄精;高尿酸血症;尿酸;黄嘌呤氧化酶
selenium-enriched Cyclocarya paliurus;Polygonatum sibiricum;hyperuricemia;uric acid;xanthine oxidase

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 罗丹;陈丹;白曦晨;向极钎;樊建元;刘卫;
LUO Dan;CHEN Dan;BAI Xichen;XIANG Jiqian;FAN Jianyuan;LIU Wei;Wuhan Bestcarrier Biotechnology Co., Ltd.;College of Life Science and Technology, Huazhong University of Science and Technology;Enshi Academy of Agricultural Sciences;Enshi Savant Ecological Agriculture Development Co., Ltd.;National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology;

参考文献(References)：

• [1] 张媛，徐红.复方芪苓配方颗粒治疗大鼠高尿酸血症的作用机制研究[J].中国现代应用药学，2020,37(15):1825-1829.
• [2] 寇惠娟，马瑞，高登峰，等.高血压患者高尿酸血症与肾损害及血浆致动脉硬化指数的关系[J].岭南心血管病杂志，2016,22(4):442-445.
• [3] 张妍，李瑶，赵亚妮，等.芒果苷、山柰酚和栀子苷对高尿酸血症小鼠的作用[J].西北药学杂志，2021,36(2):215-219.
• [4] Abeles A M,Pillinger M H.The CARES scare:Febuxostat and the risk of cardiovascular disease [J].Arthritis Rheumatol,2019,71(6):1023-1024.
• [5] 唐梅，赵立春，扈芷怡，等.青钱柳化学成分及药理作用研究进展[J].国际药学研究杂志，2017,44(9):851-859.
• [6] Jin M L,Lu Z Q,Huang M,et al.Effects of Se-enriched polysaccharides produced by Enterobacter cloacae Z0206 on alloxan-induced diabetic mice[J].Int J Biol Macromol,2012,50(2):348-352.
• [7] Wang Y C,Chen J,Zhang D Z,et al.Tumoricidal effects of a selenium (Se)-polysaccharide from Ziyang green tea on human osteosarcoma U-2 OS cells[J].Carbohydr Polym,2013,98(1):1186-1190.
• [8] 陈宇，周芸湄，李丹，等.黄精的现代药理作用研究进展[J].中药材，2021,44(1):240-244.
• [9] 吕萌，陈伟鸿，许琦，等.有机富硒肥对青钱柳叶中硒、微量元素及功效成分的影响[J].农学学报，2016,6(5):39-42.
• [10] 罗丹，白曦晨，陈丹，等.富硒青钱柳-黄精复方对糖尿病大鼠糖脂代谢和肝肾功能的影响[J].今日药学，2021,31(5):353-356.
• [11] 陈丹，罗丹，白曦晨，等.富硒青钱柳-黄精复方降血糖及抗氧化作用的研究[J].特产研究，2021,43(3):11-18.
• [12] 陈丹，罗丹，白曦晨，等.富硒青钱柳-黄精复方茶饮对小鼠的免疫调节作用[J].食品安全质量检测学报，2021,12(9):3621-3626.
• [13] Palmiere C,Mangin P.Urea nitrogen,creatinine,and uric acid levels in postmortem serum,vitreous humor,and pericardial fluid[J].Int J Legal Med,2015,129(2):301-305.
• [14] 黄静，李昂，刘宇光，等.白耙齿菌固体发酵物对高尿酸血症及痛风性关节炎的治疗作用[J].西北药学杂志，2018,33(4):488-491.
• [15] 康乐，苗晋鑫，苗明三，等.芍药花总苷对小鼠高尿酸血症模型的药效机制研究[J].时珍国医国药，2020,31(8):1844-1846.