HPLC法测定龙凤宝胶囊中淫羊藿苷的含量Content Determination of Icariin in Longfengbao Capsules by HPLC
曹艳芳,黄夏敏,冯倩玲,邓海玲
CAO Yanfang,HUANG Xiamin,FENG Qianling,DENG Hailing
摘要(Abstract)：
目的建立高效液相色谱(HPLC)法测定龙凤宝胶囊中淫羊藿苷含量的方法。方法采用HPLC方法,色谱柱为Diamonsil(5μm×4.6 mm×200 mm);流动相为乙腈-水;流速1.0 m L/min;检测波长270 nm。结果淫羊藿苷在0.011 7~0.117 0mg/m L浓度范围内有良好的线性关系(r=0.999 8)。平均回收率为101.26%,RSD=2.66%。结论该方法可准确地进行龙凤宝胶囊中淫羊藿苷的含量测定,可用于该制剂的质量控制。
OBJECTIVE To establish the HPLC method for the determination of Icariin in Longfengbao capsules.METHODS HPLC was performed on Diamonsil( 5 μm×4.6 mm×200 mm). The mobile phase was acetonitrile-water. The detection wavelength was270 nm,and the flow rate was 1.0 m L / min.RESULTS Icariin showed a good linearity in the range of 0.011 7 ~ 0.117 0 mg / m L,r =0.999 8. The average recovery was 101.26%,and RSD was 2.66%. CONCLUSION This method is effective and can be used for the quality control of Icariin in Longfengbao capsules.
关键词(KeyWords)：
龙凤宝胶囊;淫羊藿苷;高效液相色谱法;含量测定
Longfengbao capsules;Icariin;HPLC;determination
基金项目(Foundation):
作者(Author):
曹艳芳,黄夏敏,冯倩玲,邓海玲
CAO Yanfang,HUANG Xiamin,FENG Qianling,DENG Hailing
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