唐细兰,宋湘芝,陈子和,陈冬萍
Tang Xilan;Song Xiangzhi;Chen Zihe;Chen Dongping (The Third Affiliated Hospital of Sun Yat sen University of Medical Sciences;

• 1:中山医科大学附属第三医院
• 2:中山医科大学附属第三医院
• 3:广东药学院
• 4:广东药学院 广州510630现工作单位:中山医科大学附属中山眼科医院

摘要(Abstract)：

目的 :建立HPLC法同时测定血清中 4种苯二氮类药物硝西泮、艾司唑仑、阿普唑仑、地西泮的浓度。方法 :血清样品在碱性条件下用乙醚 (已去除过氧化物 )提取 ,4 0℃水浴蒸干 ,残渣用流动相溶解 ,进样分析。色谱条件为DiscoveryC18柱 5 μm (2 5 0mm× 4 6mm) ,流动相为甲醇∶乙腈∶水 (42∶18∶4 0V/V ) ,检测波长2 30nm ,柱温 4 0℃。结果 :4种药物一次性分离 ,方法回收率均为 97 7～ 98 5 % ,r >0 .999。日内 ,日间RSD均不大于 7%。结论 :本方法简便快速 ,适用于临床血药浓度监测及此类药物中毒的快速定性定量的分析。
Objective:To establish a rapid and simple HPLC method for the simultaneous determination of 4 kinds of benzodiazepines including nitrazepam,estazolam,alprazolam and diazepam in serum.Method:The serum samples were extracted with ethyl ether,and the residue was reconstituted in methanol.The mabile phase consisted of methanol acetonitrile water(42∶18∶40 v/v).A discovery C 18 column (250mm×4.6mm,5μm) was used.The detection wavelength was 230nm.Results:4 drugs were separated simultaneously.The coefficients of the linear equation was 0.999.The recovery ratio was 97.74～98.49%,RSD of intra day and inter day are less than 7%.Conclusion:The method was simple and rapid.It was suitable for rapid qualitative and quantitative determination of the toxic drugs as well as the therapeutic monitoring of patients in the serum.

关键词(KeyWords)： 苯二氮类药物;血药浓度;高效液相色谱法
Benzodiazepines;Serum concentration;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 唐细兰,宋湘芝,陈子和,陈冬萍
Tang Xilan;Song Xiangzhi;Chen Zihe;Chen Dongping (The Third Affiliated Hospital of Sun Yat sen University of Medical Sciences;

参考文献(References)：

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