王立军,陈榕
WANG Li-jun;CHEN Rong (Peking University Shenzhen Hospital;

• 1:北京大学深圳医院
• 2:北京大学深圳医院 广东 深圳
• 3:518036
• 4:广东 深圳

摘要(Abstract)：

目的 建立测定氯霉素滴眼液含量的方法。方法 采用紫外分光光度法,以水为空白,检测波长279 nm。结果 氯霉素在251-50μg/ml浓度范围内具有良好的线性关系。平均回收率99.4％,RSD是0.33％。与中国药典方法相比差异无显著性。结论 方法简便、快速、准确、线性范围较宽,可用于测定氯霉素滴眼液含量。
Objective To establish a method for determining Chloramphenicol eye drops. Methods UV spectrophotometry was used, λ =279nm. Results The linesr range was from 25 -50 μg/ml,mean recovers was 99.4% . There were no significant differences between ChP method and UV spectrophotometry method. Conclusion The method is simple, rapid, and accurate for the determination of chloramphenicol eye drops .

关键词(KeyWords)： 氯霉素滴眼液;含量测定;紫外分光光度法;微生物检定法
Chloramphencil Eye Deops; determination; UV Spetrophotometry; microbial assay

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基金项目(Foundation):

作者(Authors): 王立军,陈榕
WANG Li-jun;CHEN Rong (Peking University Shenzhen Hospital;

参考文献(References)：

• [1] 国家药典委员会.中国药典[S].北京:化学工业出版社,2000． 附录XIA,81．
• [2] 张守尧,王桂芳,刘楚峰.双波长分光光度法测定氯雌油中氯霉素的含量[J].广东药学,2000,10(1) :34．