今日药学

2021, v.31(06) 422-428

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网络药理学方法研究三消饮干预糖尿病的机制
Study on Action Mechanism of Sanxiaoyin in the Intervention of Diabetes Mellitus by Network Pharmacology

吴冰心;周大标;肖瑞;郑莉;
WU Bingxin;ZHOU Dabiao;XIAO Rui;ZHENG Li;Hubei University of Chinese Medicine;Xinhua Hospital of Hubei University of Chinese Medicine,Hubei Provincial Hospital of Integrated Chinese and Western Medicine Wuhan;

摘要(Abstract):

目的采用网络药理学方法探索三消饮治疗糖尿病及其并发症的作用机制。方法借助中药系统药理学分析平台(TCMSP)检索三消饮组方中草果、槟榔、厚朴、白芍、甘草、知母、甘草、黄芩、大黄、葛根、羌活、柴胡的活性成分及作用靶点。以Uniprot数据库对靶点名称进行统一规范并找到靶点对应的基因,进而运用Cytoscape 3.7.2构建药物-成分-靶点-疾病网络图,以STRING绘制蛋白相互作用的PPI网络图,最后基于R软件使用Bioconductor生物信息软件包进行关键靶基因GO与KEGG富集分析,探索药物活性成分的作用途径,将结果以条形图形式和气泡图形式进行可视化。结果三消饮共筛选出206种活性成分,药物靶标242个,疾病靶点1 068个,筛选出三消饮治疗糖尿病的相关靶点107个,GO富集结果中相关生物过程2 028条、细胞组分63条、分子功能127条,KEGG富集结果有169条相关通路。分析结果表明,三消饮活性成分中槲皮素、山柰酚、异鼠李素等与糖尿病及其并发症的治疗相关性最强。结论三消饮中的活性成分能通过调节VEGFA、Akt1、NOS3、MAPK等多种物质及介入氧化应激、HIF-1α信号通路、Toll样受体信号通路等多种途径来干预糖尿病的发展和进程,从而发挥对糖尿病及其并发症的治疗作用。
OBJECTIVE To explore the mechanism of Sanxiaoyin in treating diabetes and its complications by means of network pharmacology. METHODS The active components and action targets of Tsaoko,Areca catechu,Magnolia officinalis,Radix Paeoniae Alba,Glycyrrhrizae Radix,Rhizoma Anemarrhenae,Glycyrrhrizae Radix,Radix Scutellariae,Radix et Rhizoma Rhei,Radix Puerariae,Notopterygii Rhizoma and Bupleuri Radix in Sanxiaoyin were searched by TCM systematic pharmacological analysis platform(TCMSP). Uniprot database was used to standardize the target names and find the genes corresponding to the targets. Cytoscape 3.7.2 was used to construct the drug-component-target-disease network diagram, and the PPI network diagram of protein interaction was drawn by STRING. Finally, in order to explore the action path of active ingredients of drugs, Bioconductor bioinformatics software package based on R software was used to analyze the enrichment of key target genes GO and KEGG,and to visualize the results in the form of bar graph and bubble graph. RESULTS A total of 206 active components were screened out from Sanxiaoyin, including 242 drug targets, 1 068 disease targets, and 107 related targets for the treatment of diabetes by Sanxiaoyin. Among the enrichment results of GO,2 028 related biological processes, 63 cellular components, and 127 molecular functions were identified, while there were 169 related pathways in the enrichment results of KEGG. The analysis results showed that quercetin, kaempferol and isorhamnetin in the active components of Sanxiaoyin had the strongest correlation with the treatment of diabetes and its complications. CONCLUSION The active components in Sanxiaoyin can interfere with the development and progression of diabetes by regulating VEGFA,Akt1,NOS3,MAPK and intervening in many pathways such as oxidative stress, HIF-1α signaling pathway, Toll-like receptor signaling pathway, which plays a role in the treatment of diabetes and its complications.

关键词(KeyWords): 三消饮;糖尿病;糖尿病并发症;网络药理学;活性成分
Sanxiaoyin;diabetes;diabetic complications;network pharmacology;active components

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基金项目(Foundation): 湖北省自然科学基金(2019CFB398)

作者(Author): 吴冰心;周大标;肖瑞;郑莉;
WU Bingxin;ZHOU Dabiao;XIAO Rui;ZHENG Li;Hubei University of Chinese Medicine;Xinhua Hospital of Hubei University of Chinese Medicine,Hubei Provincial Hospital of Integrated Chinese and Western Medicine Wuhan;

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