今日药学

2021, v.31(05) 343-348

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当归挥发油对肺腺癌A549细胞增殖、迁移及细胞周期的影响
Effect of Volatile Oil of Angelica Sinensis on the Proliferation Migration and Cell Cycle of Lung Adenocarcinoma A549 Cells in Vitro

张国强;戴海蓉;高军太;
ZHANG Guoqiang;DAI Hairong;GAO Juntai;Department of Pharmacy,Pingliang City Hospital of Chinese Medicine;College of Pharmacy,Gansu University of Chinese Medicine;

摘要(Abstract):

目的研究当归挥发油对肺腺癌A549细胞增殖抑制、凋亡、迁移及周期的影响。方法体外培养肺腺癌A549细胞,采用MTT比色法检测细胞活性并计算抑制率;采用细胞划痕试验检测细胞迁移能力;采用流式细胞仪检测A549细胞凋亡及周期分布。结果相比于阴性对照组,6.25~400μg·mL~(-1)当归中挥发油对肺腺癌A549细胞增殖表现出明显的浓度依赖性抑制作用(P<0.05,P<0.01),IC_(50)值为76.17μg·mL~(-1)。当归挥发油25,50,100μg·mL~(-1)组的细胞凋亡率显著上升(P<0.01);当归挥发油50,100μg·mL~(-1)组的伤口愈合率显著降低(P<0.05,P<0.01);当归挥发油25,50,100μg·mL~(-1)组G_0/G_1、G_2/M期的细胞比例明显升高(P<0.05,P<0.01),S期细胞比例明显降低(P<0.01)。与卡铂组(25μg·mL~(-1))比较,100μg·mL~(-1)的当归挥发油对A549细胞增殖抑制、凋亡、迁移及周期的影响差异均无统计学意义(P>0.05)。结论当归挥发油可以抑制肺腺癌A549细胞的增殖,其机制可能为诱导细胞凋亡、抑制迁移,将细胞周期阻滞在G_0/G_1期与G_2/M期。
OBJECTIVE To study the effect of Angelica sinensis volatile oil on cell proliferation inhibition, apoptosis, migration and cell cycle of lung adenocarcinoma A549 cells. METHODS The lung adenocarcinoma A549 cells were cultivated in vitro.The cell activity was detected by MTT colorimetric assay and the inhibition rate was calculated.Wound healing method was used to detect the migration of A549 cells.The cell apoptosis and cycle were analyzed by flow cytometry. RESULTS Compared to the negative group, 6.25-400 μg·mL~(-1) Angelica sinensis volatile oil showed a significant concentration-dependent inhibitory effect on the proliferation of A549 cells(P<0.05,P<0.01),the IC_(50) was 76.17 μg·mL~(-1).The apoptosis rate of 25,50,100 μg·mL~(-1) Angelica sinensis volatile oil groups increased significantly(P<0.01).The wound healing rate of 50,100 μg·mL~(-1) Angelica sinensis volatile oil groups significantly decreased(P<0.05,P<0.01).The proportion of cells of the phases of G_0/G_1 and G_2/M in 12.5,25,50,100 μg·mL~(-1) volatile oil groups increased significantly(P<0.05,P<0.01),and the percentage of cells in S phase decreased significantly(P<0.01).Compared with the carboplatin group(25 μg·mL~(-1)),there was no significant difference in the effects of 100 μg·mL~(-1) volatile oil group on A549 cell proliferation inhibition, apoptosis, migration and cycle(P>0.05). CONCLUSION Volatile oil of Angelica sinensis can inhibit the viability of lung adenocarcinoma A549 cells in vitro.The mechanism may be to induce apoptosis, inhibit cell migration and arrest proliferation at G_0/G_1 and G_2/M phase.

关键词(KeyWords): 当归挥发油;肺腺癌A549细胞;细胞增殖;细胞迁移;细胞凋亡;细胞周期
volatile oil of Angelica sinensis;lung adenocarcinoma A549 cells;cell proliferation;cell migration;cell apoptosis;cell cycle

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基金项目(Foundation): 甘肃省自然科学基金项目(1508RJZA046);; 平凉市中医药文化传承与发展创新研究(PLSKKT202034)

作者(Author): 张国强;戴海蓉;高军太;
ZHANG Guoqiang;DAI Hairong;GAO Juntai;Department of Pharmacy,Pingliang City Hospital of Chinese Medicine;College of Pharmacy,Gansu University of Chinese Medicine;

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