李丽娜,林生文,朱炳辉
LI li-na,LIN Sheng-wen,ZHU Bing-hui (Guangdong Institute for Drug Control

• 1:广东省药品检验所
• 2:广东省药品检验所
• 3:广东省药品检验所 广州510180
• 4:广州510180

摘要(Abstract)：

目的采用高效液相色谱法测定巴戟口服液中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。方法以乙腈水(20∶80)为流动相,大连依利特ODS(2)柱(250mm×4.6mm,5μm)为固定相,流速为1ml/min,检测波长为320nm。结果2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的测定在0.004264~0.1066mg/ml范围内线性关系良好,平均回收率为97·9%,RSD为1.3%。结论方法简单、灵敏、快速,可用于巴戟口服液中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定。
Objective To develop a HPLC method for the assay of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-gLucoside in baji koufuye. Methods Separation was performed on a ODS(2) column(250 mm×4.6 mm,5 μm). The mobile phase consisted of acetonitrile:water(20∶80,v/v). The flow rate was 1 ml·min~ -1 . The UV detector wavelength was set at 320 nm. Result 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relation-ship in the range of 0.004264～0.1066 mg/ml, the average recovery was 97.9% with RSD 1.3 %. Conclusion The method is simple, sensitive and rapid and it can be applied to determinate the content of baji koufuye.

关键词(KeyWords)： 巴戟口服液;液相色谱法
Baji koufuye; HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李丽娜,林生文,朱炳辉
LI li-na,LIN Sheng-wen,ZHU Bing-hui (Guangdong Institute for Drug Control

参考文献(References)：

• [1]国家药典委员会.中国药典(2005年版)一部[S].北京:化学工业出版社,2005:140.
• [2]朱炳辉,梁艺英,莫金垣.固相萃取-高效液相色谱法测定海马巴戟丸中芍药苷的含量[J].中成药,1999,23(10):500.