灯盏细辛注射液体外抗菌作用及其无菌检查Antibacterial effect in vitro of injection of Erigeron brevisca Pus and its sterility test
张新妹,张癸荣,胡昌勤
Zhang Xinmei;Zhang Guirong 1; Hu Changqin (Department of Antibiotics;
摘要(Abstract)：
目的 探讨灯盏细辛注射液体外抗菌活性及其无菌检查方法。方法 应用三种药物体外抗菌、抑菌实验方法 ,检查灯盏细辛注射液体外抗菌作用并初步确定其无菌检查方法 ;通过无菌验证实验方法来确定其无菌检查方法的有效性。结果 灯盏细辛注射液的直接接种法无菌检查法抑菌实验显示其对金黄色葡萄球菌的强大抑制作用 ,直接接种法不能用于其无菌检查 ;进一步体外抗菌、抑菌实验显示其体外具有较强的抗菌、抑菌作用 ;应用薄膜过滤法对其无菌项目进行检查 ,无菌验证实验确定其无菌检查的有效方法为薄膜过滤法。结论 灯盏细辛注射液体外对金黄色葡萄球菌的显示出强大的抑制作用 ,其无菌检查的有效方法为薄膜过滤法
Objective To determine antibacterial effect in vitro of injection of Erigeron brevisca Pus and its sterility test. Methods The methods of antibacte rial effects in vitro was used to investigate the antibacterial effect of inject ion of Erigeron brevisca Pus,and the method of sterility was established by a validiation test. Results Injection of Erigeron brevisca Pu s showed strong bacteriostasis effect in vitro , as a result,direct inoculatio n method isn't fit for its sterility test,membrane filtration method was the val idatory method of its sterility test. Conclusion Injection of Erigeron brevisca Pus has strong bacteriostasis effects in vitro,the suitab le method of its sterility test is membrane filtration method.
关键词(KeyWords)：
灯盏细辛注射液;体外抗菌作用;无菌检查
Injection of Erigeron brevisca Pus; antibacterial effect in vitro;sterility test
基金项目(Foundation):
作者(Authors):
张新妹,张癸荣,胡昌勤
Zhang Xinmei;Zhang Guirong 1; Hu Changqin (Department of Antibiotics;
参考文献(References)：
- [1]张 杰,王玉琼,李永平.灯盏细辛及其相关产品应用近况[J].中国民族民间医药杂志,2001,51:191.
- [2]张卫东,陈万生,孔德云.中药灯盏细辛的化学成分研究(II)[J].第二军医大学学报,2000,21(10):914.
- [3]中华人民共和国国家药典委员会.中国药典(一部)[S].北京:化学工业出版社,2000.附录XIIIB .
- [4]马绪荣,苏德模.药品微生物学检验手册[M ].北京:科学出版社,2000.211.
- [5]邱述玲.盐酸丁卡因注射液的抑菌试验及对无菌检查方法的改进[J].中国医院药学杂志1998;18(7):323.
- [6]TheUnitedStatesPharmacopeialConvention.Inc.UnitedStatesPharmacopoeia25[S].TheUnitedStates:TheU nitedStatesPharmacopeialConvention.Inc,2001ap pendix.
- [7]王家骥,范圣凯.云南灯盏花注射液的药理研究[J].北京中医,1999,(5):64