张清民,丁扬洲,冯少斌
ZHANG Qingmin,DING Yangzhou,FENG Shaobin

• 1:广东罗浮山国药股份有限公司

摘要(Abstract)：

目的建立采用HPLC法同时测定消炎利胆片中穿心莲内酯、脱水穿心莲内酯和新穿心莲内酯的含量测定方法。方法采用HPLC法,色谱柱:Shiseido Capcell Pak-C18MGS5色谱柱(250 mm×4.6 mm,5μm)柱,以甲醇-水(52∶48)为流动相,检测波长:穿心莲内酯为225 nm,脱水穿心莲内酯为254 nm,新穿心莲内酯为210 nm。结果穿心莲内酯在线性范围0.286 7~0.009 0 mg/m L内,浓度与峰面积有良好的线性关系,RSD=0.37%,平均回收率为103.3%(n=9);脱水穿心莲内酯在线性范围0.661 8~0.020 7 mg/m L内,浓度与峰面积有良好的线性关系,RSD=0.71%,平均回收率为99.4%(n=9);新穿心莲内酯在线性范围0.343 0~0.010 7 mg/m L内,浓度与峰面积有良好的线性关系,RSD=0.83%,平均回收率为99.8%(n=9)。结论该法简便、灵敏、准确,可用于消炎利胆片的质量控制。
OBJECTIVE To develop an HPLC method for the determination of andrographolide,dehydroandrographolide,and neoandrographolide in Xiaoyan Lidan tablets.METHODS The separation was performed on a Shiseido Capcell Pak-C18MGS5 column( 250 mm×4.6 mm,5 μm) with a mobile phase of methanol-water( 52 ∶48),and the detecting wavelength was 225 nm. RESULTS The linear range of andrographolide was 0.286 7-0.009 0 mg / m L,respectively,RSD = 0.37%,and the average recovery was 103.3%( n = 9); The linear range of dehydroandrographolide was 0.661 8-0.020 7 mg/m L,respectively,RSD = 0.71%,and the average recovery was 99.4%( n = 9); The linear range of neoandrographolide was 0. 343 0- 0. 010 7 mg / m L,RSD = 0. 83%,and the average recovery was 99.8%( n = 9). CONCLUSION The method is simple,sensitive,and accurate,so it can be used to control the quality of Xiaoyan Lidan tablets.

关键词(KeyWords)： 高效液相色谱法;消炎利胆片;穿心莲内酯;脱水穿心莲内酯;新穿心莲内酯
HPLC;Xiaoyan Lidan tablets;andrographolide;dehydroandrographolide;neoandrographolide

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张清民,丁扬洲,冯少斌
ZHANG Qingmin,DING Yangzhou,FENG Shaobin

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