L-天冬酰胺酶的研究进展Advances in L-asparaginase research
吴晓英,吴振强,林影,夏枫耿
WU Xiao-ying ;WU Zhen-qiang ;LIN Ying ;X1A Feng-geng
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摘要(Abstract)：
L-天冬酰胺酶具有显著的抗肿瘤作用,尤其是广泛应用于儿童急性淋巴细胞白血病(ALL)的治疗。作者对L-天冬酰胺 酶的分离纯化工艺、与ALL的治疗、酶的修饰等进行了综述。
L-asparaginase is a very effective anti-tumor agent,it is a widely used agent against childhood acute lymphoblastic leukemia (ALL). The paper introduces L-asparaginase about its isolation and purification, about the treatment of ALL,and its medification.
关键词(KeyWords)：
L-天冬酰胺酶;儿童急性淋巴细胞白血病;分离纯化;修饰
L-asparaginase; childhood acute lymphoblastic leukemia; isolation and purification, modification
基金项目(Foundation): 广州市科学基金资助(项目编号:2002 Z3-D0181)。
作者(Authors):
吴晓英,吴振强,林影,夏枫耿
WU Xiao-ying ;WU Zhen-qiang ;LIN Ying ;X1A Feng-geng
;
参考文献(References)：
- [1] Kidd JC, Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum[ J]. J Exp Med, 1953,98:565．
- [2] Broom JO. Dvidence that the L-asparaginase activity of guinea pig serum is responsible for its antilympKomas effects[J]. Nature, 1961,19:114．
- [3] Ravindranath Y, Abella E, Krischer JP, et al. Acute myeloid leukemia( AML) in Down's syndrome is highly responsive to chemotherapy: experience on Pediatric Oncology Croup AML STUDY 8498[J]. Blood, 1992,80:2210-2214．
- [4] 李良铸,李明华.最新生化药物制备技术[M].北京:中国医药科技出版社,2001,209-210．
- [5] 刘红,潘红春,钟世荣.大肠杆菌L-天冬酰胺酶的分离纯化及其特性[J].中国生物制品学杂志,2002,15(20:93-96．
- [6] Martin del Valle EM.Freitas SC.Calan MA. Separation of asparaginase and trypsin by affinity chromatography combined with batchwise adsorption and columnwise desorption [J]. Process Biochemistry,2002,38:551-557．
- [7] MooIa ZB,Scawen MD, Atkinson T,et al. Erwinia chrysan-themi L-asparaginase: epitope mapping and production of an-tigenically modified enzymes[J]. Blochem. J. 1994,302: 921-927．
- [8] Priest JR, Ramsay NKC,Bennett AJ,et al. The effect of L-asparaginase on antithrombin, plasminogenm, and plasma coagulation during theragy for acute lymphoblastic leukemia [J]. J Pediatr. 1982,1000:900-904．
- [9] Liebman HA , Wada JK, Patch MJ, et al. Depression of func- tional and antigenic plasma antithrombin Ⅲ ( AT-Ⅲ ) due to therapy with L-asparaginase [ J ]. Cancer, 1982,50:451-455．
- [10] Nowak GU,Boos J,Wolff JEA,et al. Asparaginase decreases clotting factors in vitro; a possible pitfall? [J]. Int J Clin Lab Res, 1995,25: 146-150．
- [11] Bushman JE, Diane P, Herbert C, et al. Insight into the mechanism of asparaginase-induced depletion of antithrombin Ⅲ in treatment of childhood acute lymphoblastic leukemia [ J]. Leukemia Research ,2000,24:559-565．
- [12] Nowak GU, Kuhn N, Wolff JEA, et al. Inhibition of hyper-coagulation by antithrombin substitution in E. coli L-aspar-aginase-treated children [ J ]. Eur J Haematol, 1996,56:35-38．
- [13] Pogliani EM , Parma M , Baragetti I ,et al. L-asparaginase in acute lymphoblastic leukemia treatment: the role of human antithrombin III concentrates in regulating the prothrombot-ic state induced by therapy[J]. Acta Haematol, 1995 ,93 :5-9．
- [14] Humphries JE. Thromophilia and complex acquired deficiencies of antithrombin, protein C, and protein S [ J ]. Se-min Hematol 1995,32:8-12．
- [15] Wang B,Hak LJ, Rolling MV,et al. ELISA to evaluate plasma anti-asparaginase IgG concentrations in patients with acute lymphoblastic leukemia [ J ]. J Immunological Methods. 2000,239:75-83．
- [16] Soares AL, Guimaraes CM, Polakiewicz B,et al. Effect of polyethylene glycol attachment on physicochemical and biological stability of E. coli L-asparaginase [ J ]. Int J pharmaceutics. 2002,237-163-170．
- [17] 史凌洋、魏东芝,用活化的单甲氧基聚乙二醇修饰L-天冬酰胺酶[J].华东理工大学学报,2001,27(12) :601-604．