段夏菲,郭朝阳,段冀江
DUAN Xiafei,GUO Chaoyang,DUAN Jijiang

• 1:广州市海珠区疾病预防控制中心
• 2:广州市海珠区素社街社区卫生服务中心

摘要(Abstract)：

目的采用高效液相色谱法测定芷冰鼻腔原位凝胶中阿魏酸的含量。方法采用Agilent HC-C18(5μm,4.6 mm×250mm)为色谱柱;流动相为甲醇-1%醋酸溶液(30∶70);流速为1 m L/min;检测波长为321 nm;柱温为20℃。结果阿魏酸浓度在10~50μg/m L范围内呈现良好的线性关系,平均加样回收率为99.2%,RSD为1.00%(n=6)。结论本法简便、准确,可作为该制剂的含量控制方法。
OBJECTIVE To determine the Ferulic acid in Zhibing in-situ gel nasal-cavity by HPLC. METHODS The Agilent HC-C18( 4. 6 mm × 250 mm,5 μm) was adopted as the chromatographic columns; Methanol-1% acetate solution( 30∶ 70) was used as the mobile phase; the flow rate was 1 m L / min; detection wavelength was 321 nm; the column temperature was 20 ℃. RESULTS The amount of ferulic acid in the sample was linear in the range of 10-50 μg / m L,the average recovery was 99. 2%,and RSD was 1. 00%( n = 6). CONCLUSION This method was simple and accurate,and can be used for the content control of the preparation.

关键词(KeyWords)： 芷冰鼻腔原位凝胶;阿魏酸;HPLC;含量测定
Zhibing in-situ gel nasal-cavity;ferulic acid;HPLC;content determination

Abstract:

Keywords:

基金项目(Foundation): 广州市医药卫生科技项目(20131A011178);; 广州市海珠区科技计划项目(2012-yl-20)

作者(Author): 段夏菲,郭朝阳,段冀江
DUAN Xiafei,GUO Chaoyang,DUAN Jijiang

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