高效液相色谱法测定栀子及其炮制品中熊果酸的含量Determination of ursolic acid in Fructus Gardenies and its prepared medical materials by HPLC
梁迎
Liang Ying (Guangdong Yanling Hospital;
摘要(Abstract)：
目的 建立栀子及其炮制品中熊果酸的测定法,考察栀子炮制前后熊果酸的含量变化。方法 采用高效液相色谱法,色谱柱为Hypersil C_(18)5μm(4.6mm×250mm),流动相为0.02mol/L NaH_2PO_4(pH2.6):甲醇(16:84),检测波长220mm。结果 在23～135μg/ml范围内,呈良好的线性关系,平均加样回收率和RSD分别为97.81％、1.51％。栀子炮制后熊果酸含量下降。结论 该方法可用于栀子及炮制品的质量控制,炮制可导致栀子中熊果酸的含量下降。
Objective To establish a HPLC method for the determination of ursolic acid in Fructus Gardeniae and study the quantitative change after prepared. Methods HPLC method was used. The column was Hypersil C18 5 um(4. 6 mm x 250 mm). The mobil phase was 0.02 mol/L NaH2PO4 (pH2. 6) :methanol(16: 84). The detective wavelength was 220 mm. Results The average recovery was 97,81% ,RSD 1.51%. Conclusion The method was sample and accurate. It can be used for the quality control of Fructus Gardeniae. Roast could lead to quantitative decrease of ursolic acid in Fructus Gardeniae.
关键词(KeyWords)：
栀子;熊果酸;高效液相色谱法
Fructus Gardeniae; ursolic acid;HPLC
基金项目(Foundation):
作者(Authors):
梁迎
Liang Ying (Guangdong Yanling Hospital;