李咏梅,周小忠,钟淑娟,罗思婧,江伟鹏
LI Yongmei,ZHOU Xiaozhong,ZHONG Shujuan,LUO Sijing,JIANG Weipeng

• 1:广州医科大学附属第五医院
• 2:广州花海药业股份有限公司
• 3:广州固志药物研究有限公司

摘要(Abstract)：

目的建立高效液相色谱法(HPLC)测定花地灌肠液中芦丁的含量。方法色谱柱为Thermo ODS-2(4.6 mm×250 mm,5μm),流动相为甲醇-1%甲酸(32∶68),检测波长为257 nm,流速为1.0 m L/min。结果芦丁线性范围为0.827 4~4.137μg(r=0.999 9),平均回收率为99.7%(RSD=1.0%)。结论该方法简便、快速、分离度好、结果稳定,可用于花地灌肠液的质量控制。
OBJECTIVE To establish an HPLC method for the determination of rutin in Huadi enema. METHODS The separation was performed on Thermo ODS-2(4. 6 mm × 250 mm,5 μm) column with Methanol- 1% formic acid(32∶ 68) as a mobile phase,the detection wavelength was at 257 nm,and the flow rate was 1. 0 m L / min. RESULTS The linear range of rutin was within0. 827 4- 4. 137 μg( r = 0. 999 9). The average recovery of rutin was 99. 7%( RSD = 1. 0%). CONCLUSION The method is simple,rapid and capable of producing good separation results. It can be applied to the quality control of Huadi enema.

关键词(KeyWords)： 高效液相色谱法;花地灌肠液;芦丁;含量
HPLC;Huadi enema;rutin;content

Abstract:

Keywords:

基金项目(Foundation): 建设中医药强省立项资助科研课题(20141181)

作者(Author): 李咏梅,周小忠,钟淑娟,罗思婧,江伟鹏
LI Yongmei,ZHOU Xiaozhong,ZHONG Shujuan,LUO Sijing,JIANG Weipeng

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典[S].一部.中国医药科技出版社,2010:333-334.
• [2]皮晓华,邓桂明.高效液相色谱法测定槐榆丸中芦丁含量的研究[J].中国现代药物应用,2007,1(11):14-15.