黄晓娟,黄丽洋,丁建军,胡建恩,陈玮,杨杰,许青松,麻攀
HUANG Xiao-juan,HUANG Li-yang,DING Jian-jun,HU Jian-en,CHEN Wei,YANG Jie,XU Qing-song,MA Pan(1.Dalian Fisheries University

• 1:大连海洋大学
• 2:中科院大连化学物理研究所

摘要(Abstract)：

目的探讨珠蛋白寡肽对猪主动脉血管内皮细胞(PAEC)的内皮素-1(ET-1)分泌水平的影响。方法本研究采用ELISA方法。结果猪主动脉内皮细胞在正常培养下,内皮素的含量是18.1μg/ml。珠蛋白寡肽组分Ⅱ、Ⅲ、Ⅳ、Ⅴ作用48h后,内皮素含量分别减少了13.9%,13.1%,15.2%,17.7%。结论研究表明珠蛋白胃蛋白水解产物,经SephadexLH-20柱层析分离后得到的5个寡肽组分中,组分Ⅱ、Ⅲ、Ⅳ、Ⅴ对PAEC的ET-1的分泌有抑制作用。
Objective To determine the effect of globin oligopeptide on porcine aortic endothelial cells(PAEC) of endothelin1(ET-1) secretion levels.Methods ELISA method was used.Results The endothelin content was 18.1 μg/ml while the porcine aortic endothelial cells under normal culture.After the globin oligopeptide components Ⅱ,Ⅲ,Ⅳ,Ⅴwas cultured for 48 h,the level of ET decreased by 13.9%,13.1%,15.2%,17.7%.Conclusion Among 5 oligopeptide components that obtained after peptone hydrolyzates of globin protein chromatographic separated by SephadexLH-20 column,the components Ⅱ,Ⅲ,Ⅳ,Ⅴ can inhibit ET-1secretion of the PAEC.

关键词(KeyWords)： 珠蛋白;寡肽;血管内皮细胞;内皮素-1;酶标免疫
globin;oligopeptide;endothelial cell;endothelin-1;ELISA

Abstract:

Keywords:

基金项目(Foundation): 国家“八六三”计划项目资助(编号:2006AA10Z331)

作者(Author): 黄晓娟,黄丽洋,丁建军,胡建恩,陈玮,杨杰,许青松,麻攀
HUANG Xiao-juan,HUANG Li-yang,DING Jian-jun,HU Jian-en,CHEN Wei,YANG Jie,XU Qing-song,MA Pan(1.Dalian Fisheries University

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