今日药学

2014, v.24(03) 164-167

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高表达SUMO蛋白对人肝星状细胞LX-2纤维化的影响
Effect of Overexpressing SUMO Protein on Fibrogenesis in Human Hepatic Stellate Cell Line LX-2

潘裕华;许晨舒;李樱媚;曹聪;陈孝;
PAN Yu-hua;XU Chen-shu;LI Ying-mei;CAO Cong;CHEN Xiao;Department of Pharmacy,the First Affiliated Hospital,Sun Yat-sen University;

摘要(Abstract):

目的研究在体外培养的人肝星状细胞LX-2中高表达SUMO蛋白对LX-2纤维化指标的影响。方法采用脂质体瞬时转染法分别将SUMO-1/2/3的高表达质粒转染入LX-2细胞中,分别运用RT-PCR和Western Blot检测α-SMA、CollagenⅠ、MMP-2、TIMP-1的mRNA和蛋白表达量。结果转染质粒48 h后α-SMA、CollagenⅠ的mRNA和蛋白表达水平显著降低(P<0.05),MMP-2、TIMP-1的mRNA表达量亦显著降低(P<0.01),但MMP-2和TIMP-1的蛋白表达水平无明显影响。结论高表达SUMO蛋白能抑制人肝星状细胞LX-2的纤维化过程。
Objective To investigate the effect of overexpression of SUMO protein on fibrogenesis in Human Hepatic Stellate Cell Line LX-2 in vitro. Methods LX-2 cells were transfected with SUMO-1 /2 /3 overexpressing plasmids. Real-time PCR and Western Blot were used to measure the mRNA and protein expression of α-SMA,CollagenⅠ,MMP-2 and TIMP-1, respectively. Results The mRNA and protein expression of α-SMA and CollagenⅠwere significantly reduced 48 h after transfection of SUMO plasmids. The mRNA expression of MMP-2 and TIMP-1 was also markedly downregulated. However,the protein expression of MMP-2 and TIMP-1 was not affected. Conclusion Overexpression of SUMO protein inhibited the expression of fibrogenesis in LX-2 cells.

关键词(KeyWords): SUMO;肝星状细胞;肝纤维化
SUMO protein;hepatic stellate cells;hepatic fibrosis

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基金项目(Foundation): 广东省药学会研究基金(编号:2013A25);; 广东省重大科技专项(编号:2011A080300004)

作者(Author): 潘裕华;许晨舒;李樱媚;曹聪;陈孝;
PAN Yu-hua;XU Chen-shu;LI Ying-mei;CAO Cong;CHEN Xiao;Department of Pharmacy,the First Affiliated Hospital,Sun Yat-sen University;

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