谢健鸣,隆颖
XIE Jianming,LONG Ying

• 1:广东省食品药品检验所

摘要(Abstract)：

目的采用HPLC法测定头痛灵胶囊中芍药苷的含量。方法采用高效液相色谱法。色谱柱为Venusil XBP C18(250mm×4.6 mm,5μm);以乙腈-0.05%磷酸溶液(14∶86)为流动相,流速为1.0 m L/min,柱温25℃,检测波长为230 nm。结果芍药苷在0.172 34~1.723 4μg范围内呈良好的线性关系(r=1),平均回收率为101.8%,RSD为0.9。结论该方法操作易行、结果可靠、重复性好,可用于头痛灵胶囊的质量控制。
OBJECTIVE To establish a method for the determination of paeoniflorin in Toutongling capsules by HPLC.METHODS HPLC was performed on a Venusil XBP C18column( 250 mm × 4. 6 mm,5 μm). The mobile phase was acetonitrile-0.05% phosphate( 14 ∶ 86),and flow rate was 1. 0 m L/min,column temperature 25 ℃. The detection wavelength was 230 nm.RESULTS The paeoniflorin had a good linearity in the ranges of 0.172 34- 1.723 4 μg( r = 1). The average recovery was 101.8%with RSD = 0.9%.CONCLUSION The method is for the determination of easy operation,reliable results,and reproducible. It can be used for the quality control of paeoniflorin in Toutongling capsules.

关键词(KeyWords)： HPLC;头痛灵胶囊;芍药苷
Toutongling capsules;HPLC;paeoniflorin

Abstract:

Keywords:

基金项目(Foundation): 广东省中医药局科研课题(20142028)

作者(Author): 谢健鸣,隆颖
XIE Jianming,LONG Ying

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社,2010,231.
• [2]王模强,张会.丹栀逍遥颗粒的含量测定方法研究[J].海峡药学,2014,26(3):72-74.
• [3]石小鹏,缪珊,毕琳琳.芪生胶囊中芍药苷的含量测定[J].医药导报,2014,33(7):937-939.
• [4]纪开明.铜陵和亳州牡丹皮中丹皮酚和芍药苷的含量比较研究[J].中医药导报,2014,20(10):78-80.