HPLC测定西咪替丁胶囊的含量Determination of Cimetidine Capsules by HPLC
李蔚,陈日檬,万晓明
LI Wei,CHEN Ri-meng,WAN Xiao-ming (Zhanjiang Institute for Drug Control
摘要(Abstract)：
目的建立西咪替丁胶囊的含量测定方法。方法采用W aters高效液相色谱仪,TIANHE C18柱(250 mm×4.6 mm,10μm),0.05 mo.lL-1磷酸二氢钾溶液(三乙胺调节pH至5.8)-甲醇(210∶300)为流动相,检测波长为219 nm测定西咪替丁胶囊的含量。结果使用高效液相色谱法可以准确有效地测定西咪替丁胶囊的含量。结论该方法在5~50μg.m l-1的浓度范围内线性关系良好(r=0.9999,n=6),平均回收率为100.3%,RSD=0.6%(n=6),方法简便,准确,适用于西咪替丁胶囊的含量测定。
Objective To establish an HPLC method for determination of cemitidine capsules.Method The determination was performed on a TIANHE C_(18) reversed column(250 mm ×4.6 mm,10 μm).The mobile phase was 0.05 mol·L~(-1) Potassium dihydrogen phosphate(adjusted to pH 5.8 with triethylamine)-methanol(210∶300).The detection wavelength was set at 219 nm.Result and conclusion The assay was linear from 5～50 μg·ml~(-1)(r=0.9999,n=6),the average recovery was 100.3%,RSD=0.6%(n=6).The method is simple,convenient and accurate and is suitable for the detemination of Cimetidine capsules.
关键词(KeyWords)：
西咪替丁;高效液相色谱法
cimetidine;HPLC
基金项目(Foundation):
作者(Author):
李蔚,陈日檬,万晓明
LI Wei,CHEN Ri-meng,WAN Xiao-ming (Zhanjiang Institute for Drug Control