梁洪,李波,刘艳芳,冯丽洁,宋艳刚
LIANG Hong,LI Bo,LIU Yanfang,FENG Lijie,Song Yangang

• 1:丽珠集团利民制药厂

摘要(Abstract)：

目的建立测定田参氨基酸胶囊中药类成分含量的高效液相色谱(HPLC)法,以全面有效控制田参氨基酸胶囊质量。方法采用Phenomenex Kinetex C_(18)色谱柱(100 mm×4.6 mm,2.6μm);流动相:乙腈-水,梯度洗脱,流速:0.8 mL·min~(-1);检测波长:203 nm;柱温:30℃。结果三七皂苷R_1、人参皂苷Rg_1、Re、Rb_1、Rd线性范围分别为0.060 9~0.811 6,0.237 2~3.162 8,0.237 2~3.162 8,0.038 7~0.526 0,0.226 9~3.012 0,0.226 9~3.012 0和0.058 1~0.774 8 mg·mL~(-1)(r≥0.999 8);阴性无干扰;精密度、稳定性、重复性良好;回收率在93%~103%范围内。结论建立的方法准确、简便、重复性好,可用于田参氨基酸胶囊的质量控制。
OBJECTIVE To establish a method for the determination of the content of the traditional Chinese medicine components in the Notoginsenoside-Ginseng amino acid capsules. METHODS The column was Phenomenex Kinetex C18column( 100 mm×4.6 mm,2.6 μm); with mobile phase of acetonitrile/water( gradient elution) at a flow rate of 0.8 mL·min~(-1),the detection wavelength was 203 nm,column temperature was 30 ℃. RESULTS The linear range was 0. 060 9-0. 811 6 mg·mL~(-1) for notoginsenoside R1,0.237 2-3.162 8 mg·mL~(-1) for ginsenoside Rg_1,0.038 7-0.526 0 mg·mL~(-1) for ginsenoside Re,0.226 9-3.012 0 mg·mL~(-1) for ginsenoside Rb1,0.058 1-0.774 8 mg·mL~(-1) for ginsenoside Rd. RSDs of precision,stability and reproducibility tests were lower than 3%,recoveries were 93%-103%. CONCLUSION The established HPLC method is accurate,simple and reproducible,and can be used for the quality control of Notoginsenoside-Ginseng amino acid capsules.

关键词(KeyWords)： 田参氨基酸胶囊;三七皂苷R_1、人参皂苷Rg_1、Re、Rb_1、Rd;高效液相色谱法
Notoginsenoside-Ginseng amino acid capsules;notoginsenoside R_1,ginsenoside Rg_1,Re,Rb_1,Rd;HPLC

Abstract:

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基金项目(Foundation):

作者(Author): 梁洪,李波,刘艳芳,冯丽洁,宋艳刚
LIANG Hong,LI Bo,LIU Yanfang,FENG Lijie,Song Yangang

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