何洋,肖伟娟,曹演威,乔明福
HE Yang1,XIAO Wei-juan1,CAO Yan-wei1,QIAO Ming-fu2 (1.Guangdong Pharmocutical University Zhongshan campus

• 1:广东药学院中山校区
• 2:珠海联邦制药股份有限公司

摘要(Abstract)：

目的建立以高效液相色谱法(HPLC)同时测定枳实中3种黄酮类成分含量的方法。方法色谱柱为Waters XBridge C18柱(4.6mm×150mm,5μm),流动相为乙腈-甲醇-0.2%磷酸溶液(18∶1∶81,v/v/v),流速为1.0ml/min,检测波长为283nm,柱温为室温。结果柚皮苷、橙皮苷和新橙皮苷的浓度分别在0.6000～14.40μg/ml、0.2180～5.232μg/ml和0.6900～16.56μg/ml范围内与峰面积呈良好的线性关系,相关系数分别为0.9995、0.9996和0.9996;平均回收率分别为101.1%、100.8%和100.5%,相对标准偏差值(RSD)分别为1.0%、0.9%和0.7%。结论本方法简便、快捷,结果准确,重复性好,可作为枳实的质量控制方法之一。
Objective To establish an HPLC method for the content determination of three flavonoids in Fructus Aurantii Immaturus. Methods The analytical method was carried out with XBridge C18 (4.6 mm×150 mm,5 um) column and the mobile phase consisted of acetonitrile-methanol-0.2% phosphoric acid solution(18∶1∶81,v/v/v) as a flow rate of 1.0 ml/min.The detection wavelength was 283 nm. Results The calibration curve was linear within the range of 0.6000～14.40 μg/ml(r = 0.9995),0.2180～5.232 μg/ml(r = 0.999 6) and 0.6900～16.56 μg/ml (r = 0.999 6) for naringin,hesperidin and neohesperidin,respectively. The average recoveries were 101.1 %(RSD = 1.0 %),100.8 %(RSD = 0.9 %) and 100.5 %(RSD = 0.7 %)for naringin,hesperidin and neohesperidin,respectively. Conclusion The method is simple,accurate,reproducible and precise,and applicable for the determination of three flavonoids in Fructus Aurantii Immaturus.

关键词(KeyWords)： 枳实;柚皮苷;橙皮苷;新橙皮苷;高效液相色谱法
Fructus Aurantii Immaturus;Naringin;Hesperidin;Neohesperidin;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 何洋,肖伟娟,曹演威,乔明福
HE Yang1,XIAO Wei-juan1,CAO Yan-wei1,QIAO Ming-fu2 (1.Guangdong Pharmocutical University Zhongshan campus

参考文献(References)：

• 1国家药典委员会.中华人民共和国药典.北京:化学工业出版社,2005.172.
• 2蔡逸平,曹岚,范崔生.枳实、积壳药材的化学成分及药理研究概况.江西中医学院学报,1999,11(1):l8.