廖华卫,李瑞珍,陈飞苑
LIAO Hua-wei~1,LI Rui-zhen~2,CHEN Fei-yuan~2 (1.Guangdong College of Pharmacy

• 1:广东药学院中药学院
• 2:中山大学附属第三医院药剂科
• 3:中山大学附属第三医院药剂科 广州510006
• 4:广州510630

摘要(Abstract)：

目的研究该颗粒剂中麻黄和甘草薄层色谱鉴别的方法。方法麻黄的鉴别:硅胶G薄层板,氯仿-甲醇-氨试液(9∶4∶0.20)为展开剂,茚三酮试液为显色剂;甘草酸的鉴别:硅胶GF254(不活化),正丁醇-浓氨试液-水-乙醇(13∶1∶4∶2)为展开剂,紫外灯(254 nm)下检视;甘草次酸的鉴别:硅胶G薄层板,石油醚-苯-乙酸乙酯-冰乙酸(10∶22∶6∶0.45)为展开剂,喷以10%磷钼酸乙醇液。结果与结论本文所提供的方法专属性强,可用于冷哮丸的质量控制。
Objective To study the TLC identification of Herba Ephedrae and Radix Glycyrrhizae in the preparation.Methods The identification of Herba Ephedrae: silica gel G plate,Chloroform-methanol-ammonia water(9∶4∶0.20) as developing reagent,ninhydrin as color developing rengent.The identification of Glycyrrhizic acid: silica gel GF254(no activation),Nbutanol-ammonia water-water-ethanol(13∶1∶4∶2) as developing reagent,inspected in ultraviolet light(254 nm). The identification of Glycyrrhetinic acid: silica gel G plate,petroleum-ether-benzene-glacial acetic acid(10∶22∶6∶0.45) as developing reagent,10%phosphomolybdic acid ethanol solution as color developing reagent.Result and Conlusion This method had good specificity and can be used in the quality control of preparation.

关键词(KeyWords)： 薄层色谱;麻黄;甘草
TLC;Herba Ephedrae;Radix Glycyrrhizae

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 廖华卫,李瑞珍,陈飞苑
LIAO Hua-wei~1,LI Rui-zhen~2,CHEN Fei-yuan~2 (1.Guangdong College of Pharmacy

参考文献(References)：

• [1]黄泰康.常用中药成分与药理手册[M].北京:中国医药科技出版社,1994:1624.
• [2]李光喜,邓红,张蜀.青梅感冒冲剂中的岗梅和甘草的薄层色谱鉴别[J].广东药学院学报,2003,19(2):107-108.