黄少愉,吴学昊,黄远翔,洪燕春,陈灿林,简惠莹,王瑶,范华均
HUANG Shaoyu,WU Xuehao,HUANG Yuanxiang,HONG Yanchun,CHEN Canlin,JIAN Huiying,WANG Yao,FAN Huajun

• 1:广东药学院药科学院

摘要(Abstract)：

目的建立微波辅助-双水相萃取苦参多糖的方法,并优化其萃取实验条件。方法以乙醇/硫酸铵双水相为萃取溶剂,利用单因素试验和正交试验考察了双水相体系的组成、萃取温度、萃取时间、液料比等因素对香菇多糖萃取的影响。结果微波萃取苦参多糖的最佳萃取条件为:双水相体系组成为19%硫酸铵(w/w)和25%乙醇(w/w),萃取温度为80℃,萃取时间25 min,液料比70∶1。经过3次验证实验,苦参多糖的总平均萃取率为13.97%。结论微波辅助-双水相萃取苦参多糖简单、快速,高效,而且可利用双水相的分相作用和极性差异萃取不同的苦参多糖;该方法可作为苦参多糖萃取和样品前处理方法。
OBJECTIVE To establish microwave-assisted aqueous two-phase extraction( MAATPE) of polysaccharides from Sophora flavescens Ait. with ethanol ammonium sulfate system, and to optimize its extraction conditions. METHODS The experimental factors including extraction temperature,extraction time,solvent-to-material ratio,ethanol concentration,ammonium sulfate concentration were investigated by single-factor test and orthogonal tests. RESULTS The optimum conditions of MAATPE were as follows: aqueous two-phase system( ATPS) consisted of 19% ammonium sulfate and 25% ethanol,ratio solvent-to-material of70∶ 1,extraction temperature of 80 ℃,and extraction time of 25 min. The total extraction yield of triplicate validations for polysaccharides was up to 13. 97 %( w / w) under the optimum conditions. CONCLUSION MAATPE is a green,rapid and efficient alternative to extraction of polysaccharides from Sophora flavescens Ait. It is also used as sample pretreatment for analysis of polysaccharides from natural products.

关键词(KeyWords)： 微波辅助萃取;双水相萃取;苦参;多糖;正交试验
microwave-assisted extraction;aqueous two-phase extraction;Sophora flavescens Ait.;polysaccharides;orthogonal tests

Abstract:

Keywords:

基金项目(Foundation): 2014年广东药学院学生课外科技项目(49)

作者(Author): 黄少愉,吴学昊,黄远翔,洪燕春,陈灿林,简惠莹,王瑶,范华均
HUANG Shaoyu,WU Xuehao,HUANG Yuanxiang,HONG Yanchun,CHEN Canlin,JIAN Huiying,WANG Yao,FAN Huajun

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