林航;周炳;李映娜;彭毅侯;周浩强;陈智;张薇;范华均;
LIN Hang;ZHOU Bing;LI Yingna;PENG Yihou;ZHOU Haoqiang;CHEN Zhi;ZHANG Wei;FAN Huajun;College of Pharmacy,Guangdong Pharmaceutical University;School of Basic Course,Guangdong Pharmaceutical University;

• 1:广东药学院药科学院
• 2:广东药学院基础学院

摘要(Abstract)：

目的建立密闭微波辅助-双水相萃取方法萃取香菇多糖方法并优化其萃取条件。方法采用单因素和正交试验方法考察了双水相组成、萃取温度、萃取时间、液料比等因素对香菇多糖萃取的影响。结果微波辅助-双水相萃取香菇多糖的优化条件为:硫酸铵质量百分数为19%,乙醇质量百分数为26%组成双水相,萃取温度为80℃,萃取时间30 min,液料比50∶1,经过验证香菇多糖的总平均萃取率为13.66%。结论微波辅助-双水相萃取香菇多糖方法具有快速,高效,选择性高的特点,可作为香菇多糖萃取和样品前处理方法。
OBJECTIVE To establish a novel method of pressurized microwave-assisted aqueous two-phase extraction( PMAATPE) of polysaccharides from Lentinula edodes,and to optimize its extraction condition. METHODS The influence of experimental factors including extraction temperature,extraction time,solvent-to-material ratio,ethanol concentration,ammonium sulfate concentration on the extraction of polysaccharides from Lentinula edodes were investigated by single-factor test and orthogonal tests.RESULTS The optimum condition of PMAATPE were aqueous two-phase system( ATPS) consisted of 19% ammonium sulfate and 26% ethanol,ratio solvent-to-material of 50 ∶1,extraction temperature of 80 ℃,and extraction time of 30 min. The total average yield of validation test for Lentinula edodes polysaccharides was up to 13.66%( w / w). CONCLUSION PMAATPE is a green,rapid and efficient alternative to extraction polysaccharides from Lentinula edodes with higher selectivity.

关键词(KeyWords)： 微波辅助双水相萃取;香菇;多糖;正交试验
microwave-assisted aqueous two-phase extraction;Lentinula edodes;polysaccharide;orthogonal tests

Abstract:

Keywords:

基金项目(Foundation): 广东药学院大学生创新创业训练计划项目(201410573043)

作者(Authors): 林航;周炳;李映娜;彭毅侯;周浩强;陈智;张薇;范华均;
LIN Hang;ZHOU Bing;LI Yingna;PENG Yihou;ZHOU Haoqiang;CHEN Zhi;ZHANG Wei;FAN Huajun;College of Pharmacy,Guangdong Pharmaceutical University;School of Basic Course,Guangdong Pharmaceutical University;

参考文献(References)：

• [1]张军霞,胡喜巧.香菇多糖几种萃取方法的比较[J].河南科技学院学报,2008,3:45-49.
• [2]林俊,李萍,陈靠山.近5年多糖抗肿瘤活性研究进展[J].中国中药杂志,2013,4:1116-1125.
• [3]Bisen PS,Baghel RK,Sanodiya BS,et al.Lentinus edodes:a macrofungus with pharmacological activities[J].Current Medicinal Chemistry,2010,17(22):2419-2430.
• [4]孟思彤,徐艳芝,王振月.多糖的化学修饰对其生物活性影响研究进展[J].天然产物研究与开发,2014,26:1901-1905.
• [5]Ma CQ,Xia R,Peng Y,et al.A study of methods for extraction of lentinan and amino acids from the foot of lentinus edodes.Herbal Med,2003,22:372-374.
• [6]Chihara G,Hamuro J,Maeda YY,et al.Fractionation and purification of the polysaccharides with marked antitumor activity,especially lentinan,from Lentinus edodes(Berk.)Sing.(an edible mushroom)[J].Cancer Research,1970,30(11):2776-2781.
• [7]游丽君,邹林武,梁彦豪,等.超声-高温热水萃取香菇多糖及其产物特性研究[J].现代食品科技,2013,29(9):2167-2172.
• [8]张楷正,杨跃寰,李光辉,等.香菇多糖萃取方法研究进展[J].四川理工学院学报:自然科学版,2009,22(6):63-66.
• [9]申进文,冯雅岚,庄庆利,等.香菇子实体多糖萃取工艺优化研究[J].河南农业科学,2010(3):90-92.
• [10]Zhang W,Zhu D,Fan HJ,et al.Simultaneous extraction and purification of alkaloids from Sophora Flavescens Ait.by microwave-assisted aqueous two-phase extraction with ethanol/ammonia sulfate system[J].Separation and Purification Technology,2015,141:113-123.
• [11]Luo Y,Pan XG,Liu G,et al.Optimization of the way coloration in the process of determination polysaccharide content by pheolsulfuric acid method[J].Chin J Inf TCM,2005,12:45-46.
• [12]徐海军,孙稹稹.应用双水相萃取技术萃取香菇多糖的研究[J].天然产物研究与开发,2013,25:982-985,911.
• [13]江秀娟,梁华伦,陈燕霞,等.小驳骨总黄酮含量测定及提取工艺研究[J].今日药学,2014,24(4):258-261.
• [14]陈吉生,吕剑豪.超声法提取枸杞多糖工艺研究[J].今日药学,2009,19(21):46-48,51.
• [15]关恺珍,程轩轩,张旭红,等.响应面法优化龙眼核中总黄酮的提取工艺[J].今日药学,2014,24(1):18-22.
• [16]张薇,陈浩浩,刘小琴,等.不同微波辅助法提取苦参生物碱的比较[J].中国实验方剂学杂志,2012,18(20):34-38.