今日药学

2015, v.25(08) 564-568

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正交试验优化密闭微波辅助-双水相萃取香菇多糖的研究
Optimization of Pressurized Microwave-Assisted Aqueous Two-Phase Extraction of Polysaccharides from Lentinula Edodes with Orthogonal Test

林航;周炳;李映娜;彭毅侯;周浩强;陈智;张薇;范华均;
LIN Hang;ZHOU Bing;LI Yingna;PENG Yihou;ZHOU Haoqiang;CHEN Zhi;ZHANG Wei;FAN Huajun;College of Pharmacy,Guangdong Pharmaceutical University;School of Basic Course,Guangdong Pharmaceutical University;

摘要(Abstract):

目的建立密闭微波辅助-双水相萃取方法萃取香菇多糖方法并优化其萃取条件。方法采用单因素和正交试验方法考察了双水相组成、萃取温度、萃取时间、液料比等因素对香菇多糖萃取的影响。结果微波辅助-双水相萃取香菇多糖的优化条件为:硫酸铵质量百分数为19%,乙醇质量百分数为26%组成双水相,萃取温度为80℃,萃取时间30 min,液料比50∶1,经过验证香菇多糖的总平均萃取率为13.66%。结论微波辅助-双水相萃取香菇多糖方法具有快速,高效,选择性高的特点,可作为香菇多糖萃取和样品前处理方法。
OBJECTIVE To establish a novel method of pressurized microwave-assisted aqueous two-phase extraction( PMAATPE) of polysaccharides from Lentinula edodes,and to optimize its extraction condition. METHODS The influence of experimental factors including extraction temperature,extraction time,solvent-to-material ratio,ethanol concentration,ammonium sulfate concentration on the extraction of polysaccharides from Lentinula edodes were investigated by single-factor test and orthogonal tests.RESULTS The optimum condition of PMAATPE were aqueous two-phase system( ATPS) consisted of 19% ammonium sulfate and 26% ethanol,ratio solvent-to-material of 50 ∶1,extraction temperature of 80 ℃,and extraction time of 30 min. The total average yield of validation test for Lentinula edodes polysaccharides was up to 13.66%( w / w). CONCLUSION PMAATPE is a green,rapid and efficient alternative to extraction polysaccharides from Lentinula edodes with higher selectivity.

关键词(KeyWords): 微波辅助双水相萃取;香菇;多糖;正交试验
microwave-assisted aqueous two-phase extraction;Lentinula edodes;polysaccharide;orthogonal tests

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基金项目(Foundation): 广东药学院大学生创新创业训练计划项目(201410573043)

作者(Author): 林航;周炳;李映娜;彭毅侯;周浩强;陈智;张薇;范华均;
LIN Hang;ZHOU Bing;LI Yingna;PENG Yihou;ZHOU Haoqiang;CHEN Zhi;ZHANG Wei;FAN Huajun;College of Pharmacy,Guangdong Pharmaceutical University;School of Basic Course,Guangdong Pharmaceutical University;

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