林晓辉,张雪原,朱吉士,郑婧婧,黄冬梅
LIN Xiaohui,ZHANG Xueyuan,ZHU Jishi,ZHENG Jingjing,HUANG Dongmei

• 1:潮州市食品药品检验所

摘要(Abstract)：

目的建立脾约丸中厚朴酚与和厚朴酚的含量测定方法。方法采用HPLC法测定脾约丸中厚朴(厚朴酚与和厚朴酚)的含量,色谱柱为Thermo BDS HYPERSIL C18,5μm,4.6 mm×250 mm,流动相为甲醇-水(75∶25),检测波长为294 nm,柱温25℃。结果和厚朴酚在6.056~90.84μg/m L的范围内、厚朴酚在6.704~100.56μg/m L的范围内呈良好线性关系,脾约丸中和厚朴酚平均回收率为99.58%(RSD为1.4%,n=9),厚朴酚平均回收率为99.62%(RSD为1.3%,n=9)。结论所建立的HPLC方法专属性强,重复性好,可用于脾约丸的质量控制。
OBJECTIVE To establish an HPLC method for determination of Magnolol and Honokiol in Piyue pill. METHODS The Thermo BDS HYPERSIL C18 was used( 5 μm,4. 6 mm×250 mm). The mobile phase consisted of methanol-water( 75 ∶25) at 25 ℃.The detection wavelength was 294 nm. RESULTS Honokiol showed a good liner relationship in the range of 6. 056-90. 84 μg / m L. The average recovery was 99. 58%( RSD = 1. 4%,n = 9). Magnolol showed a good liner relationship in the range of 6. 704-100. 56 μg / m L.The average recovery was 99. 62%( RSD = 1. 3%,n = 9). CONCLUSION The method is accurate,reliable and available with goo reproducibility. It can be used for the quality control of Piyue pill effectively.

关键词(KeyWords)： 脾约丸;厚朴酚;和厚朴酚;高效液相色谱法
Piyue pill;magnolol;honokiol;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 林晓辉,张雪原,朱吉士,郑婧婧,黄冬梅
LIN Xiaohui,ZHANG Xueyuan,ZHU Jishi,ZHENG Jingjing,HUANG Dongmei

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