鱼腥草破壁饮片指纹图谱研究Study on HPLC Fingerprint of Cell Wall-Broken Decoction Pieces of Houttuynia Cordata Thunb
沈浔,王慧玲,彭丽华,成金乐
SHEN Xun,WANG Huiling,PENG Lihua,CHENG Jinle
摘要(Abstract)：
目的建立鱼腥草破壁饮片HPLC指纹图谱,为鱼腥草破壁饮片整体质量评价提供依据。方法以十八烷基硅烷键合硅胶为填充剂的色谱柱(4.6 mm×250 mm,5μm),柱温30℃,流速1.2 m L/min,检测波长326 nm,流动相为乙腈-0.1%的磷酸溶液,梯度洗脱。结果建立了鱼腥草破壁饮片的HPLC指纹图谱,得到了7个共有特征峰,12批样品的相似度达0.94以上,方法学考察结果符合指纹图谱技术要求。结论所建立的方法稳定、可靠、重复性好,可用于鱼腥草破壁饮片质量控制和综合评价。
OBJECTIVE To establish the HPLC fingerprint of cell wall-broken decoction pieces of Houttuynia cordata Thunb,so as to provide basis for quality standard. METHODS RP-HPLC was performed on a C18column( 250 mm × 4. 6 mm,5 μm),the column temperature was set at 30 ℃,with speed of 1. 2 m L / min,while the detective wavelength was set at 326 nm,with a gradient elution composed of acetonitrile-0.1% phosphoric acid mixed solution. RESULTS The HPLC fingerprint of cell wall-broken decoction pieces of Houttuynia cordata Thunb was established,and 7 common peaks were found. The HPLC fingerprints of 12 samples were more than 0. 946 similarity measurement. The results of method validation met technical standard of fingerprints. CONCLUSION The method has good precision,stability and repeatability,and it can be used for quality control for cell wall-broken decoction pieces of Houttuynia cordata Thunb.
关键词(KeyWords)：
鱼腥草破壁饮片;HPLC;指纹图谱
cell wall-broken decoction pieces of Houttuynia cordata Thunb;HPLC;fingerprint chromatograms
基金项目(Foundation): 中山市科技计划项目(2013A3FC0253)
作者(Author):
沈浔,王慧玲,彭丽华,成金乐
SHEN Xun,WANG Huiling,PENG Lihua,CHENG Jinle
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