今日药学

2003, (05) 21-23

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

RP-HPLC梯度洗脱法测定痰咳净散剂中咖啡因和甘草酸的含量
Determination of caffeine and glycyrrhizine in Tankejing Powder by RP-HPLC gradient elution

刘劲涛,张建浩
LIU Jin-tao,ZHANG Jian-hao 1 (Guangzhou Yangcheng Pharmaceutical Co. LTD.,Guangzhou 510450,China;1.Guangdong Drug Evaluation Center,Guangzhou 510080,China)

摘要(Abstract):

目的 测定痰咳净散剂中咖啡因和甘草酸的含量。方法 在C18柱上 ,以 (A) 0 .0 1mol/LNa2 HPO4溶液 (用H3 PO4调pH为 3.0 ) ,(B)乙腈组成流动相 ,进行线性梯度洗脱 ,检测波长为 2 5 0nm ,外标法定量。 结果 线性关系良好 ,咖啡因的平均回收率为 99.7% ,RSD =0 .36 % (n =5 ) ,甘草酸的回收率为 99.6 % ,RSD =0 .4 2 % (n =5 )。结论 本法简便 ,重现性好 ,共存组分无干扰
Objective Determination of caffeine and glycyrrhizine in Tankejing Powder. Methods Discovery C 18 column,was used with a mobile phase of A (0.01 mol/L NaH 2PO 4, pH adjusted to 3.0 with phosphoric acid ), B (acetonitrile), used a linear gradient elution, detection was done at 250 nm, injection 15 μl, external standard method was adopted. Results All the results for the standards curve, the recovery and the seqaration showed that the method is satifactory. Conclution The method is sinple, rapid and reliable.

关键词(KeyWords): 痰咳净散剂;咖啡因;甘草酸
Tankejing Powder;caffeine;glycyrrhizine

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 刘劲涛,张建浩
LIU Jin-tao,ZHANG Jian-hao 1 (Guangzhou Yangcheng Pharmaceutical Co. LTD.,Guangzhou 510450,China;1.Guangdong Drug Evaluation Center,Guangzhou 510080,China)

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享