李成,徐玉红,吴芳,陈连剑,陈钦镑,魏怡雯
LI Cheng, XU Yu-hong, WU Fang, CHEN Lian-jian, CHEN Qin-bang, WEI Yi-wen (1.The Fourth people's hospital of Shenzhen

• 1:深圳市福田人民医院
• 2:深圳市福田人民医院
• 3:深圳市福田人民医院
• 4:深圳市福田人民医院

摘要(Abstract)：

目的建立复方红霉素洗剂中红霉素的含量测定方法。方法采用高效液相色谱法,色谱柱为Hypersil ODS C18柱(4.6mm×250mm,5μm),流动相为0.1mol/L磷酸二氢铵缓冲液(三乙胺调pH6.5)-乙腈(65∶35),流速为1.0ml/min,检测波长为210nm。结果红霉素在0.55~2.75mg/ml浓度范围内,峰面积与其浓度呈良好的线性关系,r=0.9992(n=5),平均回收率为99.3%,RSD为1.26%(n=6)。结论本法简便、准确、灵敏度高、重复性好,可作为复方红霉素洗剂中红霉素含量的测定方法。
Objective To establish a HPLC method for the determination of erythromycin in compound erythromycin lotion. Method The analytical column was ODS C_ 18 (250×4.6 mm,5 μm), the mobile phase was composed of 0.1 M NH_4H_2PO_4 and methanol (65∶35, v/v) at the flow rate of 1.0 ml/min, the detection wavelength was 210 nm and the column temperature was 30 ℃. Result The Calibration curve was linear within the concentration range of 0.55~2.75 mg/ml. The average recovery was 99.3%, and RSD was 1.26%(n=3). Conclusion The method is simple, rapid and accurate. It is suitable for the determination of erythromycin in compound erythromycin lotion.

关键词(KeyWords)： 复方红霉素洗剂;红霉素;高效液相色谱法;含量测定
HPLC;erythromycin;determination;lotion

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李成,徐玉红,吴芳,陈连剑,陈钦镑,魏怡雯
LI Cheng, XU Yu-hong, WU Fang, CHEN Lian-jian, CHEN Qin-bang, WEI Yi-wen (1.The Fourth people's hospital of Shenzhen

参考文献(References)：

• [1]国家药典委员会.中国药典(2005年版)二部[S].北京:化学工业出版社,2005:222.
• [2]顾斌,鲍思蔚,陆晓骅,等.紫外分光光度法测定痤疮液中红霉素的含量[J].上海医院药学,2000,11(1):45.
• [3]张家美,张湘雯,高黎黎,等.应用高效液相色谱法研究红霉素含量与微生物效价法的相关性[J].北京医科大学学报,1994.26(5):383-384.
• [4]张立军.红霉素含量的HPLC测定[J].山东医药工业,2000,19(6):13-15.
• [5]王泽明,当代结构药物全集[M].北京:科学技术出版社1993:204-205.