陈荧,张亮
CHEN Ying,ZHANG Liang

• 1:佛山市高明区人民医院药剂科
• 2:广州中医药大学

摘要(Abstract)：

目的研究依达拉奉对H_2O_2诱导HepG2细胞损伤的保护作用。方法以400μmol·L~(-1)的H_2O_2诱导HepG2细胞氧化应激损伤,MTT法测定细胞存活率,ELLSA法测定细胞中丙二醛(MDA)、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-PX)水平的变化。结果依达拉奉可降低H_2O_2引起的细胞凋亡和细胞内MDA表达水平,升高GSH-PX和T-SOD表达水平。结论依达拉奉对H_2O_2诱导的HepG2细胞氧化应激损伤有保护作用。
To investigate the protection effect of edarovane on H_2O_2 induced oxidative stress in HepG2 cells.METHODS An experimental HepG2 cell damage model induced by H_2O_2( 400μM) was established. Cell viability was measured by MTT assay. ELISA kits were used to measure GSH- PX,T-SOD activities and MDA content. RESULTS Edarovane effectively reduced the H_2O_2-induced apoptosis of HepG2 cells. Edarovane elevated the activities of superoxide dismutase( SOD) and glutathione peroxidase( GSH-PX),and the content of malondialdehyde( MDA) was decreased. CONCLUSION Edarovane shows the protection effects on H_2O_2 induced oxidative stress in HepG2 cells.

关键词(KeyWords)： 依达拉奉;H2O2;氧化应激损伤;保护作用
edaravone;H2O2;oxidative stress injury;protection

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 陈荧,张亮
CHEN Ying,ZHANG Liang

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