HPLC法测定蛋黄油中胆固醇含量Determination of Cholesterol in Egg Yolk Oil by HPLC
王小妹,刘春芳,杨阳
WANG Xiaomei,LIU Chunfang,YANG Yang
摘要(Abstract)：
目的建立高效液相色谱法测定蛋黄油中胆固醇的含量。方法样品无须皂化处理,直接用二氯甲烷-甲醇(1∶2)溶解,经反相C18色谱柱(150 mm×3.9 mm,5μm)分离后测定,以甲醇为流动相A,以甲醇-异丙醇-正已烷(10∶70∶20)为流动相B;梯度洗脱;检测波长为205 nm;流速为1.0 m L/min;柱温为35℃。结果本方法测定胆固醇线性范围为0.097 23~1.94463 mg/m L,线性方程y=3 869.446x+15.519,相关系数r=0.999 9,线性关系良好;加样回收率为96.98%~100.4%。结论该方法灵敏、快速、准确,适用于测定蛋黄油中胆固醇的含量。
OBJECTIVE To establish a method for determination of Cholesterol in egg yolk oil by HPLC. METHODS The sample was dissolved in Dichloromethane-Methanol( 1∶ 2) without saponification. The chromatographic separation was carried out on a C18( Waters C18: 150 mm × 3. 9 mm,5 μm) column using UV detector at 205 nm. Methanol and Methanol-Isopropyl alcohol-n-hexane( 10∶ 70 ∶ 20) were used as mobile phase by gradient elution. The flow rate was 1. 0 m L/min,column temperature was 35 ℃.RESULTS The linear range of Cholesterol was 0. 097 23- 1. 944 63 mg / m L,Linear equation was y = 3 869. 446 x + 15. 519( r = 0.999 9). The average recovery range was 96. 98%- 100. 4%. CONCLUSION The method is simple,quick and accurate. It can be used for determination of Cholesterol in egg yolk oil.
关键词(KeyWords)：
胆固醇;蛋黄油;高效液相色谱法;含量测定
cholesterol;egg yolk oil;HPLC;determination
基金项目(Foundation): 广州市药用脂质重点实验室项目(穗科信字[2013]163号)
作者(Author):
王小妹,刘春芳,杨阳
WANG Xiaomei,LIU Chunfang,YANG Yang
参考文献(References)：
- [1]湖南省食品药品监督管理局.湖南省中药材标准;2009年版[S].长沙,湖南科学技术出版社,2010:50-51.
- [2]王春林,唐汉钧.蛋黄油医用价值研究近况[J].中国中医药信息杂志,2005,12(6):100-102.
- [3]严建业,王璐,李顺祥,等.蛋黄油的现代研究进展[J].中国中医药信息杂志.2012,19(3):106-110.
- [4]食品中胆固醇的测定-高效液相色谱法[S].GB/T 22220-2008.
- [5]胆固醇.欧洲药典7.0版[S].1680-1681.
- [6]丁卓平,王明华,刘振华,等.食品中胆固醇含量测定方法的研究与比较[J].食品科学,2004,25(1):130-135.
- [7]房文宁,王爱国,傅金銮.不同组织样中胆固醇含量测定方法的优化[J].中国畜牧杂志,2009,45(5):48-50.
- [8]张旭,胡艳,蒋桂韬.蛋黄中胆固醇含量测定方法的研究[J].检测分析,2011(12):35-38.
- [9]陈静,刘昌勇,杜红岩,等.反相高效液相色谱法测定不同品种鸡蛋中胆固醇的含量[J].营养学报,2011,33(3):323-324.
- [10]刘佳娣,许书军,徐春祥,等.双波长分光光度法测定废弃油脂中胆固醇的含量[J].安徽农业科学,2012,40(18):9871-9873.
- [11]其布勒哈斯,李红,徐向峰,等.RP-HPLC法测定食品中胆固醇含量的研究[J].食品安全质量检测学报,2014,5(1):276-279.
- [12]廖连英,韩飞,赵质创,等.食用植物油中胆固醇含量的快速测定[J].化学分析计量,2012,21(2):32-33.