今日药学

2004, (03) 13-16

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离子对高效液相色谱法测定氨酚沙芬口服溶液中对氨基酚的量
Ion pair HPLC determination of 4-Aminophenol in Paracetamol and Dertromethorphan Hydrobromide Oral Solution

熊仁清,朱美容
XIONG Ren qing Zhongshan Dosage of The United Laboratories LTD.,Zhuhai,Zhongshan Guangdong 528467,China

摘要(Abstract):

目的 测定氨酚沙芬口服溶液中对氨基酚的量。方法 采用反相离子对色谱法。色谱柱:日本 Inertsil ODS-3 5μm4.6mm×l50mm;柱温:40℃;流动相:乙腈-0.1mol/L KH_2PO_4(内含0.03mol/L庚烷磺酸钠)溶液(取13.6gKH_2PO_4,加三乙胺2.5ml,加水1000ml,用H_3PO_4调pH值至4.0,加6.06g的庚烷磺酸钠)(10:90);检测波长:280nm;进样量:10μl。结果 测得对氨基酚的平均回收率为99.85%,RSD=0.94%。结论 方法准确可靠,适合测定氨酚沙芬口服溶液中的对氨基酚的量。
Objective To establish a HPLC method for the determination of 4-aminophenol in paracetamol and dertromethorphan hydro- bromide oral solution. Methods The column is Japan Inertsi ODS-35 μm 4.6mm×150mm. Column temperature: 40℃. The mo- bile phase composed of acetonitrile(A. C. S. HPLC)-0.03 mol/L 1-heptanesulfonic acid sodium salt in 0.1 mol/L KH_2PO_4 (transfer 13.6g KH_2 PO_4,add 2.5 ml Triethylamine,add pure water 1000 ml, adjust pH 4.0 with phosphoric acid,add 6. 06 g 1-heptanesulfonic acid sodium sall)(10:90). The detection was 280 nm. The inject volume is 10 μl. Results The average recovery of 4-aminophenol was 99. 85% (RSD=0.94%). Conclusion This method is reliable,accurte and is very suiable for the determination of 4-aminophe- nol in paracetamol and dertromethorphan hydrobromide oral solution.

关键词(KeyWords): 氨酚沙芬;口服溶液;离子对高效液相色谱法;对氨基酚
paracetamol and dertromethorphan hydrobromide;oral solution;Ion pair HPLC,4-aminophenol

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作者(Author): 熊仁清,朱美容
XIONG Ren qing Zhongshan Dosage of The United Laboratories LTD.,Zhuhai,Zhongshan Guangdong 528467,China

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