吕竹芬,郑志华,谢清春,陈燕忠,申楼
LV Zhu-fen~1, ZHENG Zhi-hua~2,XIE Qing-chun~1,CHEN Yan-zhong~1, SHEN Lou~1 (1.Institute of Materia Medica

• 1:广东药学院药物研究所
• 2:广东省药学会
• 3:广东药学院药物研究所
• 4:广东药学院药物研究所

摘要(Abstract)：

目的建立高效液相色谱法测定Beagle犬体内罗格列酮血药浓度的方法。方法以十八烷基键合硅胶柱为固定相,以0.01mol/L的磷酸二氢钾水溶液-乙腈(50∶50),以三乙胺调节pH6.0为流动相,检测波长247nm。4-硝基-2-苯氧基甲烷磺酰苯胺为内标,样品用乙醚提取,按内标法定量。结果标准曲线50~5000ng/ml范围内有良好线性关系(r=0.9996),最低检测浓度为30ng/ml,平均方法回收率为97.34%(RSD为2.15%)。结论本文建立的方法能够准确测定罗格列酮的血药浓度,线性关系好,回收率高,稳定耐用。
Objective To establish a assay method for the determination of rosiglitazone in serum of Beagle dogs. Method Analysis was conducted with the column of C_8 (250×4.6 mm, 5 μm) and mobile phase of 0.01 mol/L potassium dihydrophosphate-acetonitrile (50∶50) (pH value was adjusted to 6.0 with triethylamine),and rosiglitazone was determined at the wavelength of 247 nm., and 4-nitro-2-phenoxymethane sulfonanilide as interal standard. Results In the concentration range of 50～5000 ng·ml~ -1 , the distribution of rosiglitazone is linear (r=0.9996), with an average recovery of 97.34%(RSD was 2.15%).Conclusion The assay method is convenient, accurate, and reliable with good precision, and can be used in the determination of rosiglitazone in serum of beagle dog.

关键词(KeyWords)： 罗格列酮;血药浓度;HPLC
rosiglitazone; HPLC; serum concentration

Abstract:

Keywords:

基金项目(Foundation): 广州市科技计划项目-科技攻关重点项目(项目编号:2003E2-E4062)

作者(Author): 吕竹芬,郑志华,谢清春,陈燕忠,申楼
LV Zhu-fen~1, ZHENG Zhi-hua~2,XIE Qing-chun~1,CHEN Yan-zhong~1, SHEN Lou~1 (1.Institute of Materia Medica

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