今日药学

2012, v.22(04) 219-221

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HPLC法测定益肾养元合剂中补骨脂素和异补骨脂素的含量
Determination of Psoralen and Isopsoralen in Yishen Yangyuan Heji by HPLC

侯勤;彭惠炫;李春芳;
HOU Qin,PENG Hui-xuan,LI Chun-fang(Guangzhou Pangaoshou Pharmaceutical CO.,LTD,Guangzhou,Guangdong 511480,China)

摘要(Abstract):

目的建立HPLC法测定益肾养元合剂中补骨脂素和异补骨脂素的含量。方法采用AgilentC18柱(150 mm×4.6 mm,5μm)为色谱柱;流动相为甲醇∶水(35∶65);检测波长为246 nm;流速1.0 ml/min;柱温35℃。结果补骨脂素在40.04~800.80 ng范围内有良好的线性关系(r=0.999 95),平均回收率为98.3%,RSD为2.45%;异补骨脂素在40.88~817.60 ng范围内有良好的线性关系(r=0.999 96),平均回收率为99.1%,RSD为2.16%;结论本法简单、准确可靠,可用于该制剂的质量控制。
Objective To esablish a HPLC method for determining the content of psoralen and isopsoralen in Yishen Yangyuan Heji.Methods The Agilent C18 column(150 mm×4.6 mm,5 μm) was used.The mobile phase was consisted by methanol-water(35∶65).The detection wavelength was 246 nm.The flow rate was 1.0 ml/min.column at 35 ℃.Results The lingear range of psoralen was 40.04800.80 ng(r=0.999 95).The average recovery was 98.3%,RSD was 2.45%.The lingear range of isopsoralen was 40.88800.80 ng(r=0.999 95).The average recovery was 98.3%,RSD was 2.45%.The lingear range of isopsoralen was 40.88817.60 ng(r=0.999 96).The average recovery was 99.1%,RSD was 2.16%.Conclusion The method is simple,reliable,accurate and can be applied to the quality control of the preparetion.

关键词(KeyWords): 益肾养元合剂;补骨脂素;异补骨脂素;HPLC
Yishen Yangyuan Heji;psoralen;isopsoralen;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 侯勤;彭惠炫;李春芳;
HOU Qin,PENG Hui-xuan,LI Chun-fang(Guangzhou Pangaoshou Pharmaceutical CO.,LTD,Guangzhou,Guangdong 511480,China)

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