高效液相色谱法测定癣湿药水中蛇床子素的含量Determination of osthole in Xuanshiyaoshui by HPLC
黄美兰,王希,黄荣林
Huang Meilan; Wang Xi; Huang Ronglin' (Guangdong Pharmaceutical School;
摘要(Abstract)：
目的 测定癣湿药水中蛇床子素的含量。方法 采用HPLC法,固定相为Spherisorb C_(18),流动相为甲醇-水(75:75,V/v),流速1.0ml/min;紫外检测器,波长322nm。结果 线性范围为0.6～1.4μg,r=0.9990,平均回收率为97.36%,RSD为1.37%(n=6)。结论 该法快速、精确,可用于癣药水中蛇床子素含量的测定。
Objective To determine the osthole in Xuanshiyaoshui by HPLC. Methods Stationary phase was spherisorb C18( 10um) , mobile phase consisted of methol and water(75:25) , The flow rate was 1. 5 ml/min; Detection was performed with ultralet detector at 322 nm. Results The linear range of quantity was 0. 6 - 1.4 ug. The average recovery was 97.36% ,RSD 1. 37% (n =6). Conclusion This method is rapid and accurate. It can be applied to determining the osthole in Xuanshiyaoshui.
关键词(KeyWords)：
癣湿药水;蛇床子素;高效液相色谱法
Xuanshiyaoshui; osthole; HPLC
基金项目(Foundation):
作者(Authors):
黄美兰,王希,黄荣林
Huang Meilan; Wang Xi; Huang Ronglin' (Guangdong Pharmaceutical School;
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