今日药学

2022, v.32(03) 211-214

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罗浮山百草油小鼠灌胃给药急性毒性研究
Luofushan Herbal Oil Oral Toxicity Test in Mice

黄炜忠;毛鑫;滕云霞;张曼娜;
HUANG Weizhong;MAO Xin;TENG Yunxia;ZHANG Manna;Guangdong Luofushan Sinopharm Co.,Ltd.;

摘要(Abstract):

目的 观察罗浮山百草油(LHO)单次灌胃给药可能引起的毒性反应。方法 选用KM小鼠,将LHO使用茶油稀释后(3.4~20.0 mL·kg(-1))灌胃给药,溶媒对照组灌胃给予茶油;单次给药后观察动物临床症状,行为变化、死亡情况,并进行大体解剖观察。结果 LHO给药后出现不同程度的活动增加、步态不稳、呼吸困难、阵挛性抽搐等毒性症状。给药后24 h,高剂量组(4.8~20.0 mL·kg(-1))灌胃给药,溶媒对照组灌胃给予茶油;单次给药后观察动物临床症状,行为变化、死亡情况,并进行大体解剖观察。结果 LHO给药后出现不同程度的活动增加、步态不稳、呼吸困难、阵挛性抽搐等毒性症状。给药后24 h,高剂量组(4.8~20.0 mL·kg(-1))小鼠出现死亡,各给药组存活小鼠毒性症状均消失;给药后14 d内,存活小鼠精神状态较好,外观未见异常表现,行为活动正常,9.8 mL·kg(-1))小鼠出现死亡,各给药组存活小鼠毒性症状均消失;给药后14 d内,存活小鼠精神状态较好,外观未见异常表现,行为活动正常,9.8 mL·kg(-1)及以上剂量小鼠体质量增长缓慢。死亡小鼠以及试验结束时仍存活的小鼠,大体解剖肉眼观察未见明显病变及异常。结论 LHO单次灌胃给药对小鼠的半数致死量LD_(50)=5.819 mL·kg(-1)及以上剂量小鼠体质量增长缓慢。死亡小鼠以及试验结束时仍存活的小鼠,大体解剖肉眼观察未见明显病变及异常。结论 LHO单次灌胃给药对小鼠的半数致死量LD_(50)=5.819 mL·kg(-1),相当于人的LD_(50)为0.924 mL·kg(-1),相当于人的LD_(50)为0.924 mL·kg(-1)
OBJECTIVE To observe the possible toxic reactions caused by a single oral administration of Luofushan Herbal Oil(LHO). METHODS KM mice were selected to gavage by LHO which was diluted with tea seed oil(3.4-20.0 mL·kg(-1)。
OBJECTIVE To observe the possible toxic reactions caused by a single oral administration of Luofushan Herbal Oil(LHO). METHODS KM mice were selected to gavage by LHO which was diluted with tea seed oil(3.4-20.0 mL·kg(-1)).The solvent control group was atomized tea seed oil.After a single dose of administration, the animals' clinical symptoms, behavioral changes, deaths, and perform gross anatomical observations were conducted. RESULTS After administration of LHO,toxic symptoms such as increased activity, unsteady gait, difficulty breathing, and clonic convulsions were caused.24 hours after the LHO administration, some mice in the high-dose group(4.8-20.0 mL·kg(-1)).The solvent control group was atomized tea seed oil.After a single dose of administration, the animals' clinical symptoms, behavioral changes, deaths, and perform gross anatomical observations were conducted. RESULTS After administration of LHO,toxic symptoms such as increased activity, unsteady gait, difficulty breathing, and clonic convulsions were caused.24 hours after the LHO administration, some mice in the high-dose group(4.8-20.0 mL·kg(-1)) died, the toxic symptoms of the surviving mice in each administration group disappeared.Within 14 days after the LHO administration, the surviving mice were in good mental state and showed no abnormal appearances, normal behaviors, and slow weight gain of mice with a dose of 9.8 mL·kg(-1)) died, the toxic symptoms of the surviving mice in each administration group disappeared.Within 14 days after the LHO administration, the surviving mice were in good mental state and showed no abnormal appearances, normal behaviors, and slow weight gain of mice with a dose of 9.8 mL·kg(-1) and above.The dead mice and the mice that were still alive at the end of the experiment were grossly dissected and no obvious abnormalities were found. CONCLUSION The median lethal dose(LD_(50)) of a single intragastric administration of LHO in mice is 5.819 mL·kg(-1) and above.The dead mice and the mice that were still alive at the end of the experiment were grossly dissected and no obvious abnormalities were found. CONCLUSION The median lethal dose(LD_(50)) of a single intragastric administration of LHO in mice is 5.819 mL·kg(-1),equivalent to LD_(50) of human is 0.924 mL·kg(-1),equivalent to LD_(50) of human is 0.924 mL·kg(-1).

关键词(KeyWords): 罗浮山百草油;毒性;灌胃给药;单次给药
Luofushan herbal oil;toxicity;intragastric administration;single administration

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 黄炜忠;毛鑫;滕云霞;张曼娜;
HUANG Weizhong;MAO Xin;TENG Yunxia;ZHANG Manna;Guangdong Luofushan Sinopharm Co.,Ltd.;

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