黄炜忠;毛鑫;滕云霞;张曼娜;
HUANG Weizhong;MAO Xin;TENG Yunxia;ZHANG Manna;Guangdong Luofushan Sinopharm Co.,Ltd.;

• 1:广东罗浮山国药股份有限公司

摘要(Abstract)：

目的 观察罗浮山百草油(LHO)单次灌胃给药可能引起的毒性反应。方法 选用KM小鼠，将LHO使用茶油稀释后(3.4～20.0 mL·kg^(-1))灌胃给药，溶媒对照组灌胃给予茶油；单次给药后观察动物临床症状，行为变化、死亡情况，并进行大体解剖观察。结果 LHO给药后出现不同程度的活动增加、步态不稳、呼吸困难、阵挛性抽搐等毒性症状。给药后24 h,高剂量组(4.8～20.0 mL·kg(-1))灌胃给药，溶媒对照组灌胃给予茶油；单次给药后观察动物临床症状，行为变化、死亡情况，并进行大体解剖观察。结果 LHO给药后出现不同程度的活动增加、步态不稳、呼吸困难、阵挛性抽搐等毒性症状。给药后24 h,高剂量组(4.8～20.0 mL·kg^(-1))小鼠出现死亡，各给药组存活小鼠毒性症状均消失；给药后14 d内，存活小鼠精神状态较好，外观未见异常表现，行为活动正常，9.8 mL·kg(-1))小鼠出现死亡，各给药组存活小鼠毒性症状均消失；给药后14 d内，存活小鼠精神状态较好，外观未见异常表现，行为活动正常，9.8 mL·kg^(-1)及以上剂量小鼠体质量增长缓慢。死亡小鼠以及试验结束时仍存活的小鼠，大体解剖肉眼观察未见明显病变及异常。结论 LHO单次灌胃给药对小鼠的半数致死量LD_(50)=5.819 mL·kg(-1)及以上剂量小鼠体质量增长缓慢。死亡小鼠以及试验结束时仍存活的小鼠，大体解剖肉眼观察未见明显病变及异常。结论 LHO单次灌胃给药对小鼠的半数致死量LD_(50)=5.819 mL·kg^(-1),相当于人的LD_(50)为0.924 mL·kg(-1),相当于人的LD_(50)为0.924 mL·kg^(-1)。
OBJECTIVE To observe the possible toxic reactions caused by a single oral administration of Luofushan Herbal Oil(LHO). METHODS KM mice were selected to gavage by LHO which was diluted with tea seed oil(3.4-20.0 mL·kg(-1)。
OBJECTIVE To observe the possible toxic reactions caused by a single oral administration of Luofushan Herbal Oil(LHO). METHODS KM mice were selected to gavage by LHO which was diluted with tea seed oil(3.4-20.0 mL·kg^(-1)).The solvent control group was atomized tea seed oil.After a single dose of administration, the animals' clinical symptoms, behavioral changes, deaths, and perform gross anatomical observations were conducted. RESULTS After administration of LHO,toxic symptoms such as increased activity, unsteady gait, difficulty breathing, and clonic convulsions were caused.24 hours after the LHO administration, some mice in the high-dose group(4.8-20.0 mL·kg(-1)).The solvent control group was atomized tea seed oil.After a single dose of administration, the animals' clinical symptoms, behavioral changes, deaths, and perform gross anatomical observations were conducted. RESULTS After administration of LHO,toxic symptoms such as increased activity, unsteady gait, difficulty breathing, and clonic convulsions were caused.24 hours after the LHO administration, some mice in the high-dose group(4.8-20.0 mL·kg^(-1)) died, the toxic symptoms of the surviving mice in each administration group disappeared.Within 14 days after the LHO administration, the surviving mice were in good mental state and showed no abnormal appearances, normal behaviors, and slow weight gain of mice with a dose of 9.8 mL·kg(-1)) died, the toxic symptoms of the surviving mice in each administration group disappeared.Within 14 days after the LHO administration, the surviving mice were in good mental state and showed no abnormal appearances, normal behaviors, and slow weight gain of mice with a dose of 9.8 mL·kg^(-1) and above.The dead mice and the mice that were still alive at the end of the experiment were grossly dissected and no obvious abnormalities were found. CONCLUSION The median lethal dose(LD_(50)) of a single intragastric administration of LHO in mice is 5.819 mL·kg(-1) and above.The dead mice and the mice that were still alive at the end of the experiment were grossly dissected and no obvious abnormalities were found. CONCLUSION The median lethal dose(LD_(50)) of a single intragastric administration of LHO in mice is 5.819 mL·kg^(-1),equivalent to LD_(50) of human is 0.924 mL·kg(-1),equivalent to LD_(50) of human is 0.924 mL·kg^(-1).

关键词(KeyWords)： 罗浮山百草油;毒性;灌胃给药;单次给药
Luofushan herbal oil;toxicity;intragastric administration;single administration

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 黄炜忠;毛鑫;滕云霞;张曼娜;
HUANG Weizhong;MAO Xin;TENG Yunxia;ZHANG Manna;Guangdong Luofushan Sinopharm Co.,Ltd.;

参考文献(References)：

• [1] 中华人民共和国卫生部.中华人民共和国卫生部药品标准中药成方制剂：第二十册[S].北京，1998:179-180.
• [2] 戴显娇，卓丽.罗浮山百草油治疗无名肿毒的临床效果[J].实用中西医结合临床，2017,17(7):97-98.
• [3] 江均良.罗浮山百草油治疗红蚂蚁咬伤的疗效观察[J].中国现代药物应用，2017,11(17):150-151.
• [4] 张建平.刮痧配合罗浮山百草油治疗肩周炎60例临床疗效观察[J].中国社区医师，2018,34(1):106,108.
• [5] 温天赋.刮痧配合罗浮山百草油治疗64例感冒临床疗效观察[J].中国农村卫生，2018(5):40-41.
• [6] 廖定华.罗浮山百草油吸入法联合加味止嗽散治疗感冒后咳嗽疗效观察[J].中国药业，2017,26(18):58-60.
• [7] 毛鑫，古淑尹，叶艺璐，等.罗浮山百草油抗炎作用机制及活性组分筛选研究[J].中国医院药学杂志，2021,41(16):1606-1611.
• [8] Mao X,Gu S,Sang H,et al.Essential Oil-Rich Chinese Formula Luofushan-Baicao Oil Inhibits the Infection of Influenza A Virus through the Regulation of NF-κB P65 and IRF3 Activation[J].Evidence-based Complementary and Alternative Medicine,2021:5547424.
• [9] 马丽，王小玉，郭爱花，等.柠檬精油对口臭相关细菌的影响及其机制初探[J].军事医学，2019,43(10):772-777,786.
• [10] 陈健芬，邓维，王一非.植物牛至精油超分子材料在口腔护理产品中的防腐抑菌能力研究[J].口腔护理用品工业，2019,29(3):10-14.
• [11] 王雪薇.黑皮油松松针精油纳米乳液的制备及其抑菌活性研究[D].哈尔滨：东北林业大学，2021.
• [12] 黄德浩，吴伟超，吴国兴，等.茶树精油抗炎抗菌功效的研究现状[J].今日药学，2018,28(3):211-215.
• [13] 蒋兆梅，杨应亮，胡必凤，等.桧木精油对实验性慢性咽炎动物模型的作用机制研究[J].广西中医药大学学报，2019,22(1):1-5.
• [14] 宋晓秋，徐亚杰，肖瀛，等.肉桂精油微胶囊对小鼠抗氧化活性与肠道菌群的影响[J].食品科学，2021,42(17):143-152.
• [15] 李大虎.甜橙精油微胶囊对肥胖型SD大鼠的减肥作用研究[D].重庆：西南大学，2019.
• [16] 邢丽媛，李慧婷，万娜，等.中药精油在临床应用中的风险控制问题分析[J].中草药，2021,52(8):2458-2464.
• [17] 张笑乐，向楠，殷中琼，等.油樟叶挥发油对兔的急性皮肤刺激、眼睛刺激和皮肤光毒性的研究[J].中国农学通报，2011,27(23):36-39.
• [18] 彭锦，唐璐，肖艳，等.崖柏精油的急性经口毒性、皮肤刺激性和致敏性实验[J].中国药师，2016,19(7):1420-1422.
• [19] 樊甜甜.真薰衣草精油和苦水玫瑰精油的安全性及功效性研究[D].上海：上海交通大学，2017.
• [20] 刘红杰，白杨，洪燕龙，等.不同提取方法制备的艾叶挥发油化学成分分析与急性肝毒性比较[J].中国中药杂志，2010,35(11):1439-1446.
• [21] 程阔菊，王晖，陈垦.薄荷醇的安全性研究进展[J].辽宁中医杂志，2010,37(2):377-380.
• [22] 丁元刚，马红梅，张伯礼.樟脑药理毒理研究回顾及安全性研究展[J].中国药物警戒，2012,9(1):38-42.
• [23] 王川.1,8-桉叶油素的安全性评价[D].雅安：四川农业大学，2013.