今日药学

2012, v.22(06) 350-352

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高效液相色谱法测定复方丹参输卵管灌注液中丹参素含量
Determination of Danshensu in Compound Danshen Tubal Perfusion by HPLC Assay

杨辉;刘佩坚;苏健芬;李寅;鲁琳;陈汉威;
YANG Hui,LIU Pei-jian,SU Jian-fen,LI Yin,LU Lin,CHEN Han-wei(Panyu Central Hospital,Guangzhou,Guangdong 511400,China)

摘要(Abstract):

目的建立高效液相色谱法测定复方丹参输卵管灌注液中丹参素含量。方法色谱柱:Inertsil C18柱(4.6 mm×250 mm,5μm);流动相:甲醇-1%冰醋酸,梯度洗脱;流速:1.0 ml/min;检测波长:280 nm。结果丹参素检测浓度在8.5~42.5μg/ml范围内与峰面积呈良好的线性关系(0.999 9);平均加样回收率为98.36%,RSD为1.02%。结论该方法简单、灵敏、准确,可用于复方丹参输卵管灌注液的质量控制。
Objective To establish an HPLC method for the determination of danshensu in compound danshen tubal perfusion.Methods Inertsil C18(4.6 mm×250 mm,5 μm) column was gradient eluted with mobile phase consisted of methanol-1% glacial acid(10∶90) at flow rate of 1.0 ml/min.And the detection wavelength was set at 280 nm.Results The linear range of danshensu was 8.5~42.5 μg/ml(r=0.999 9) with an average recovery of 98.36%(RSD=1.02%,n=6).Conclusion It is a simple,rapid and accurate assay which could be applied to the quality control of Compound danshen Tubal Perfusion.

关键词(KeyWords): 复方丹参输卵管灌注液;丹参素;高效液相色谱法;含量测定
Compound Danshen tubal perfusion;danshensu;HPLC;determination

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作者(Author): 杨辉;刘佩坚;苏健芬;李寅;鲁琳;陈汉威;
YANG Hui,LIU Pei-jian,SU Jian-fen,LI Yin,LU Lin,CHEN Han-wei(Panyu Central Hospital,Guangzhou,Guangdong 511400,China)

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