罗强,温莉珍,龙晓英,司徒冰
LUO Qiang,WEN Lizhen,LONG Xiaoying,SITU Bing

• 1:广州医科大学附属第三医院药学部
• 2:广州中医药大学第一附属医院药学部
• 3:广东药科大学中药学院

摘要(Abstract)：

目的研究D-101大孔吸附树脂富集纯化留兰香(Mentha spicata L.)总黄酮的最佳工艺条件,为留兰香总黄酮的进一步开发利用奠定基础。方法以留兰香总黄酮解吸率及回收率作为评价指标,采用单因素试验对D-101大孔吸附树脂的吸附容量、水洗量及上样时药液浓度、洗脱乙醇溶液浓度与用量、洗脱速率及径高比等参数进行优化。结果 D-101大孔吸附树脂富集纯化留兰香总黄酮的最佳工艺条件为:上样药液浓度0.16 g·mL~(-1)(按生药量计),药材量与D101大孔树脂质量之比为1∶4.8(g∶g),径高比1∶7,上样速率为2 BV·h~(-1)(动态吸附后静置20 min),水洗量4 BV(2 BV·h~(-1)),乙醇洗脱浓度为70%,洗脱体积为3 BV(2 BV·h~(-1))。结论该工艺经济、稳定、可行,可为留兰香进一步开发利用提供参考。
OBJECTIVE To optimise the enrichment and purification process of total flavonoids from Mentha spicata L.by D-101 macroporous adsorption resin,in order to further develpoment and utilization of total flavonoids from Mentha spicata L. METHODS With the desorption rate and recovery rate of total flavonoids as evaluation indexes,the purification process was optimized by single factor experiment through investigating the experimental parameters such as adsorption capacity,the column of washing and sample,ethanol concentration and volume for elution,flow rate of elution,ratio of diameter to height. RESULTS The optimum enrichment and purification process parameters were as follows: the concentration of sample solution was 0. 16 g·mL~(-1)( equivalent as crude drug),the amount of resin was 4.8 times of the medicinal materials,the ratio of diameter to height was 1 ∶7,the absorptive flow rate was 2 BV·h~(-1)( standing time being 20 min),washing with 4 BV water( 2 BV·h~(-1)) and eluting with 3 BV 70% ethanol( 2 BV·h~(-1)). CONCLUSION The optimum purification process is economical,stable and feasible,and can provide reference for the develpoment of Mentha spicata L.

关键词(KeyWords)： 留兰香;总黄酮;大孔吸附树脂
Mentha spicata L.;total flavonoids;macroporous adsorption resin

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 罗强,温莉珍,龙晓英,司徒冰
LUO Qiang,WEN Lizhen,LONG Xiaoying,SITU Bing

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