余婷,邹学农,罗嘉全,黄胜,李亮平,高蔓蔓,龚铭,周治宇,陈孝
YU Ting,ZOU Xuenong,LUO Jiaquan,HUANG Sheng,LI Liangping,Gao Manman,ZHOU Zhiyu,CHEN Xiao,Department of Pharmacy

• 1:中山大学附属第一医院药学部
• 2:中山大学附属第一医院骨科研究所

摘要(Abstract)：

目的检测脂多糖(Lipopolysaccharides,LPS)诱导THP-1巨噬细胞固有免疫反应不同时间点(4、8、12、24、48 h)差异表达的microRNAs(miRNAs)及预测的靶基因的表达情况,探讨骨修复材料移植早期的宿主免疫炎症反应调控机制。方法 LPS处理由佛波醇PMA诱导分化的THP-1细胞,分别在刺激4、8、12、24、48 h之后提取总RNA,用实时荧光定量PCR方法检测microRNAs表达。生物信息学预测miR-10b靶基因是SCARB2并检测其蛋白表达。结果 miR-10b、let-7家族以及miR-181c与芯片结果相符。SCARB2预测为miR-10b的靶基因,且蛋白表达上升。结论 miR-10b可能通过靶基因SCARB2参与调控炎症免疫过程。
OBJECTIVE To investigate the expression of differential expressed microRNAs and the predicted target gene in Lipopolysaccharides( LPS)-induced innate immune response of THP-1 macrophages at different time points( 4,8,12,24,48 h).And to explore the host immuno-inflammatory response regulatory mechanisms of bone grafting at early stage. METHODS The differentiated THP-1 macrophage that induced by phorbol PMA were treated by LPS,and the total RNA was extracted at 4,8,12,24,48 h,respectively. The microRNAs expression was analyzed by real time quantitative PCR methods. Bioinformatics predicted SCARB2 was the target gene of miR-10 b,and its protein expression was detected. RESULTS The expression trend of miR-10 b,let-7 families and miR-181 c was consistent with the chip results. SCARB2 was predicted as the target gene of miR-10 b,and the protein expression was aroused. CONCLUSION microRNA-10 b may be involved in regulating the immuno-inflammatory process by targeting SCARB2 gene.

关键词(KeyWords)： microRNA;固有免疫;差异表达
MicroRNAs;innate immunity;differentially expressed

Abstract:

Keywords:

基金项目(Foundation): 973基础研究项目(2012CB619100);; 国家自然科学基金重点项目(31430030)

作者(Author): 余婷,邹学农,罗嘉全,黄胜,李亮平,高蔓蔓,龚铭,周治宇,陈孝
YU Ting,ZOU Xuenong,LUO Jiaquan,HUANG Sheng,LI Liangping,Gao Manman,ZHOU Zhiyu,CHEN Xiao,Department of Pharmacy

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