曾桂雄,黄民,郭燕容,潘伟雄
Zeng Guixiong Huang min;Guo Yanrong;Pan Weixiong (Department of clinical pharmacology;Sun Yat sen university of medical sciences;

• 1:中山医科大学临床药理教研室
• 2:中山医科大学临床药理教研室
• 3:中山医科大学临床药理教研室
• 4:广东药学院 广州510080

摘要(Abstract)：

目的　建立一种检测血浆中头孢泊肟的微生物检测方法。方法　克雷伯肺炎杆菌、藤黄八叠微球菌作菌株在不同培养基及不同菌量检测血浆中头孢泊肟浓度。结果　用克雷伯肺炎杆菌 0 .0 5ml作检验定菌 ,在低 (0 .16mg/l)、中 (1.2 5mg/l)、高(5mg/l)三个浓度的回收率范围为 84.5 %～ 10 2 .9%。日内、日间差异在 5 .3%～ 7.0 %范围之内 ,室温、冻融、长期稳定性均在要求范围。标准曲线范围为 0 .0 4～ 10mg/l,相关系数r=0 .9993,方法稳定可靠。结论　此方法可用于头孢泊肟酯类药物的药动学和生物利用度研究。
objective To establish a microbiological assay method to determine plasma level of cefpodoxime. Methods Klebosiella Pneumoniae (CMCC(B)46117) and Micrococcus luteus (ATCC28001) were used and compared as the test bacteria in the determination of cefpodoxime with different culture media. Results Klebosiella Pneumoniae (CMCC(B)46117) was selected the test bacteria to determine the cefpodoxime level. The recovery range of this method was 84.5%～102.9% and the run precision (within day and between day coefficient of variation) was within 7.0% with low(0.16mg/l),middle(1.25mg/l) and high (5mg/l) cefpodoxime concentrations. This assay was shown to be linear over the cefpodoxime concentration range 0.04 to 10mg/l. Validation of this method including linearity,recovery,precision and stability met the requirement of drug determination. Conclusion A microbiologic assay method was validated and this method could be applied in the study of cefpodoxime pharmacokinetics and bioavaliability.

关键词(KeyWords)： 头孢泊肟;微生物法;克雷伯肺炎杆菌
cefpodoxime;microbiologic assay;klebostiella pneumoniae

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 曾桂雄,黄民,郭燕容,潘伟雄
Zeng Guixiong Huang min;Guo Yanrong;Pan Weixiong (Department of clinical pharmacology;Sun Yat sen university of medical sciences;

参考文献(References)：

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