果糖二磷酸锌对体外培养新生大鼠海马神经细胞生长发育的影响The effect of zinc fructose diphosphate on the growth of cultured newborn rat hippocampal neurons
陈子晟,罗焕敏
Chen Zisheng;Luo Huanmin(Department of Pharmacology;
摘要(Abstract)：
目的 研究不同剂量果糖二磷酸锌 (ZnFDP)对体外培养新生大鼠海马神经细胞的生长发育的影响。方法 采用体外培养的新生大鼠海马神经细胞 ,在血清培养液中加入低、中、高剂量ZnFDP ,使之终浓度分别为 2 .5、12 .5、12 5 μg·ml-1,通过细胞形态学观察 ,神经元突起和胞体生长状况的定量比较 ,不同培养期细胞存活数和培养液中乳酸脱氢酶 (LDH)渗漏量的测定 ,分析研究了ZnFDP对海马神经细胞生长发育的影响。结果 培养 36h后与对照组比 ,中剂量ZnFDP组海马神经元的突起数目增多 ,突起长度增加 ,胞体长径没有显著性变化 ;低剂量ZnFDP组与对照组比各项指标没有显著差别 ;高剂量ZnFDP则明显抑制神经细胞分化。培养 3d、7d、10d ,中剂量ZnFDP组细胞存活数明显高于对照组 ;培养 7d、10d ,中剂量ZnFDP组LDH渗漏量明显低于对照组。结论 一定量的ZnFDP能促进海马神经细胞的生长发育。
Objective The effects of different concentrations of zinc fructose diphosphate (ZnFDP)on the growth of newborn rat hippocampal neurons were observed by means of dissociated cell culture.Methods The newborn rat hippocampal neurons were cultured with different ZnFDP levels.The convert phase microscope was used to analyse the number and the length of neurites,as well as the maximum diameter of hippocampal neurons soma.The survival neurons count and LDH assay were carried out to investigate the effect of ZnFDP on the growth and development of neurons in different culture time.Results After 36h,the number and the length of neurites of 12.5μg·ml -1 ZnFDP group group were increased and the maximum diameter of hippocampal neurons soma did not change.The 2.5μg·ml -1 ZnFDP did not show statistical difference as compared with the control group,while 125μg·ml -1 ZnFDP inhibited notably the growth of neurons.The number of survival neurons of 12.5μg·ml -1 ZnFDP group was more than that of the control group,and the LDH activity of 12.5μg·ml -1 ZnFDP group was lower in different culture time.Conclusion This study suggested that the suitable dose of ZnFDP can promote the growth and development of hippocampal neurons.
关键词(KeyWords)：
果糖二磷酸锌;细胞培养;海马神经元
zinc fructose diphosphate;cell culture;hippocampal neurons
基金项目(Foundation):
作者(Authors):
陈子晟,罗焕敏
Chen Zisheng;Luo Huanmin(Department of Pharmacology;
参考文献(References)：
- 1任榕娜,孔祥英.海马缺锌与学习记忆.国外医学.儿科学分册,1994,21(3):139～141
- 2薛庆善,冯武鸣.神经组织的体外培养方法.解剖科学进展,1996,2(4):349～355
- 3XieXM ,SmartTG .Aphysiologicalroleforendogenouszincinrathippocampalsynapticneurotransmission.Nature,1991,349(6390):521～525
- 4刘力建,盛锡联,曹开贵,等.饲料锌对大鼠血清、海马、皮层一氧化氮含量的影响.微量元素与健康研究,1995,12(3):1～3
- 5王福,赵法.锌对新生大鼠海马神经元bcl-2mRNA表达的影响.第二军医大学学报,2000,21(3):222～225
- 6丛琳,汪之顼,李珏声,等.锌对神经细胞增殖周期DNA和蛋白质的影响.营养学报,2000,22(3):198～200