今日药学

2013, v.23(12) 794-796+802

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肾移植患者全血中环孢素A浓度检测的HPLC法和FPIA法的建立
A New Method for Monitoring Cyclosporin A in Whole Blood by HPLC and FPIA in Renal Transplant Patients

王一西;谢志红;方佳丽;叶丽卡;
WANG Yi-xi;XIE Zhi-hong;FANG Jia-li;YE Li-ka;The Second Affiliated Hospital of Guangzhou Medical University;

摘要(Abstract):

目的建立检测患者全血中环孢素A浓度的高效液相色谱法。方法血样经乙醚提取后,以C18色谱柱为固定相,乙腈-甲醇-水(72∶3∶25)为流动相,60℃柱温下进行色谱分析,紫外检测波长为210 nm。结果 CsA全血浓度在50~1 500 ng/mL范围内呈良好的线性关系,检测限为25 ng/mL,批内和批间精密度RSD均小于10%,方法的绝对回收率为60.14%~69.04%。结论 HPLC法快速准确,与FPIA法相关性良好,均可用于肾移植术后患者CsA的血药浓度监测。
Objective To develop an HPLC method for determining the concentration of cyclosporine A( CsA) in whole blood of renal transplant patients. Method Detection was performed on C 18 column with acetonitrile∶ methanol∶ water( 72∶ 3∶ 25) as mobile phase. Detecting wavelength was 210 nm,and column temperature was 60 ℃. Results The calibration curve was linear in the content rations ranged from 50 ~ 1 500 ng / mL. Absolute recovery of CsA was 60. 14% ~ 69. 04%, the inter-day and the intra-day RSD was less than 10%( n = 5). Conclusion This HPLC method is simple and reliable, as the same as FPIA method. It can be applied to determine the concentration of CsA in whole blood of patients receiving transplantation.

关键词(KeyWords): 反相高效液相色谱法;环孢素A;单克隆荧光偏振免疫法;血药浓度监测
HPLC;Cyclosporin A;FPIA;TDM

Abstract:

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基金项目(Foundation): 广州医学院博士启动项目(2009年博士启动);; 广东省医院药学研究基金(编号:2012A21)

作者(Author): 王一西;谢志红;方佳丽;叶丽卡;
WANG Yi-xi;XIE Zhi-hong;FANG Jia-li;YE Li-ka;The Second Affiliated Hospital of Guangzhou Medical University;

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