今日药学

2017, v.27(12) 793-795

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右美托咪定对脂多糖诱导HLF细胞凋亡的影响
Effects of Dexmedetomidine on LPS-induced Apoptosis in HLF Cells

张媛;谭晓明;常惠礼;
ZHANG Yuan;TAN Xiaoming;CHANG Huili;Pharmacy Department,the Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital;

摘要(Abstract):

目的探讨不同浓度右美托咪定(Dex)对脂多糖(LPS)诱导人肺成纤维样细胞(HLF)凋亡的影响。方法实验分为5组:对照组、LPS 5μg·mL~(-1)组、LPS 10μg·mL~(-1)组、Dex 0.1 nmol·L~(-1)+LPS 10μg·mL~(-1)组、Dex 1 nmol·L~(-1)+LPS 10μg·mL~(-1)组;采用Hochest染色和caspase-3活性实验检测不同浓度LPS对HLF细胞凋亡的影响。结果 LPS 5μg·mL~(-1)、LPS 10μg·mL~(-1)均能够诱导HLF细胞产生凋亡小体,并增加caspase-3活性;Dex 0.1 nmol·L~(-1)、Dex 1 nmol·L~(-1)提前作用后,凋亡小体产生减少,且caspase-3活性显著降低(P<0.05)。结论右美托咪定在0.1~1 nmol·L~(-1)的浓度范围内,能够逆转LPS诱导的HLF细胞凋亡。
OBJECTIVE To investigate the effect of dexmedetomidine on LPS-induced apoptosis in HLF cells. METHODS The experiment was divided into 5 groups: control group,LPS 5 μg·mL~(-1) group,LPS 10 μg·mL~(-1) group,Dex 0.1 nmol·L~(-1)+LPS 10 μg·mL~(-1) group,and Dex 1 nmol·L~(-1)+LPS 10 μg·mL~(-1) group.Hochest staining assay and the caspase-3 activity were used to detect the effect of LPS on apoptosis in HLF cells. RESULTS Apoptotic bodies and an increase of caspase-3 activity were observed when treated with LPS 5 μg·mL~(-1)、LPS 10 μg·mL~(-1); pretreatment of 0.1-1 nmol·L~(-1) dexmedetomidine resulted in a decrease in numbers of apoptotic bodies and caspase-3 activity( P<0.05). CONCLUSION Dexmedetomidine reversed LPS-induced apoptosis at concentrations of 0.1-1 nmol·L~(-1) in HLF cells.

关键词(KeyWords): 右美托咪定;脂多糖;凋亡
dexmedetomidine;LPS;apoptosis

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基金项目(Foundation): 广东省医院药学研究基金(2016MZ06)

作者(Author): 张媛;谭晓明;常惠礼;
ZHANG Yuan;TAN Xiaoming;CHANG Huili;Pharmacy Department,the Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital;

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