今日药学

2015, v.25(03) 156-159+162

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榄香烯联合5-FU体内外抑制人胃癌MGC80-3细胞增殖与诱导凋亡作用的研究
Antitumor Effects of Elemene Combined with Fluorouracil on Proliferation Inhibition and Apoptosis Induction in Human Gastric Cancer Cell Line MGC80-3

陈雨;王茜莎;韩重;杨威;周云;党靖涛;
CHEN Yu;WANG Xisha;HAN Zhong;YANG Wei;ZHOU Yun;DANG Jingtao;Guangdong Pharmaceutical University;Guangdong Lewwin Pharmaceutical Research Institute Co. ,Ltd.;

摘要(Abstract):

目的研究中药莪术提取物榄香烯制剂(Elemene)联合5-氟尿嘧啶(5-FU)对MGC80-3细胞增殖的抑制及诱导凋亡的作用。方法采用MTT法考察榄香烯、5-FU单药及联合作用于MGC80-3细胞的体外抗肿瘤效应;应用中效原理进行统计分析单药及联合用药对肿瘤细胞生长的影响,确定联合用药时的效应(Fa)合用指数(CI)的关系;流式细胞仪检测细胞凋亡周期改变。通过建立MGC80-3裸鼠体内异种肿瘤模型,考察榄香烯及5-FU单用及合用对荷瘤小鼠肿瘤生长的抑制作用。结果体外试验结果表明榄香烯与5-FU联合应用作用于MGC80-3细胞可产生较好协同效应,单染法(PI)检测到榄香烯与5-FU联合之后能够使MGC80-3细胞表现出显著的G0/G1期阻滞作用;体内试验结果表明榄香烯单药及与5-FU联合作用对MGC80-3裸鼠异种肿瘤生长均有明显的抑制作用,且联合用药组抑瘤率可达84.68%。结论榄香烯联合5-FU作用于MGC80-3肿瘤细胞株可产生较好的协同效应。
OBJECTIVE To investigate the antitumor effects of elemene combined with fluorouracil on the proliferation inhibition and apoptosis induction in human gastric cancer. METHODS The synergistic interaction on human gastric cancer was evaluated by using MTT and the combination index( CI) method. The median-effect principle was used to analyze the relationship of Fa-CI by drawing growth curves of alone / combination effects on tumor cells; The cell cycle and apoptosis were observed by flow cytometry. Nude mouse models of the solid tumor of gastric cancer were established. The inhibitory rates of solid tumor were observed by elemene and fluorouracil alone and in combination therapy. RESULTS Elemene appeared a promising candidate for combination with fluorouracil in cancer chemotherapy and the combination showed significant G0 / G1 arrest. Growth of cells was significantly inhibited in a dose and reaction time dependent manner which were treated with various concentrations of drug. The combination group suppressed the most obviously tumor growth. CONCLUSION When elemene combined with fluorouracil,elemene has synergetic effect in inhibiting the proliferation of gastric cancer cells.

关键词(KeyWords): 榄香烯;胃癌;中效原理;协同作用;凋亡
elemene;gastric cancer;the work principle;synergistic effect;apoptosis

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基金项目(Foundation): 国家十二五科技重大专项项目(2013ZX09103001012)

作者(Author): 陈雨;王茜莎;韩重;杨威;周云;党靖涛;
CHEN Yu;WANG Xisha;HAN Zhong;YANG Wei;ZHOU Yun;DANG Jingtao;Guangdong Pharmaceutical University;Guangdong Lewwin Pharmaceutical Research Institute Co. ,Ltd.;

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