今日药学

2017, v.27(07) 475-478+482

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白藜芦醇通过上调Hsp75蛋白表达改善Aβ对C17.2细胞增殖的抑制作用
Resveratrol Ameliorates the Inhibitory Effect of Aβ on the Proliferation of C17.2 Cells by Up-regulating the Expression of Hsp75 Protein

朱江华;范焕琼;
Zhu Jiang Hua;Fan Huan Qiong;Foshan Women and Children Hospital;Pharmacy department,The 5th People's Hospital of Foshan City;

摘要(Abstract):

目的探讨白藜芦醇拮抗Aβ_(1-42)诱导的体外培养神经干细胞C17.2凋亡的可能机制。方法实验分组分别为:空白对照组、Aβ_(1-42)(10μmol·L~(-1))处理组、白藜芦醇干预组(白藜芦醇1,5,10,20μmol·L~(-1)+Aβ_(1-42)10μmol·L~(-1))。采用MTT法检测细胞活力;流式细胞仪检测细胞凋亡率;采用Western blotting法分别检测热休克蛋白75(Hsp75)和活化型caspase-3蛋白的表达水平。结果与Aβ_(1-42)处理组相比,白藜芦醇预处理组呈剂量依赖性地显著提高神经干细胞的存活率(P<0.05)和凋亡率(P<0.05);同样地,显著上调C17.2细胞Hsp75蛋白的表达和降低其活化型caspase-3蛋白水平(P<0.05)。而单独使用白藜芦醇对正常C17.2细胞活性影响的差异无统计学意义(P>0.05)。结论白藜芦醇对Aβ_(1-42)诱导的C17.2细胞损伤具有明显保护作用,其作用机制可能与上调C17.2细胞Hsp75蛋白的表达水平和抑制caspase-3蛋白介导的凋亡途径从而降低细胞凋亡有关。
OBJECTIVE To investigate the possible mechanism of resveratrol weakening the apoptosis of neural stem cells C17.2induced by Aβ_(1-42) in vitro. METHODS In this paper,we set six experimental groups,such as control group,Aβ_(1-42)( 10 μmol·L~(-1))treating group,resveratrol pretreating group( 1,5,10,20 μmol·L~(-1)+ Aβ_(1-42)10 μmol·L~(-1)). Cell viability was measured by MTT assay;apoptotic rate was detected by Flow cytometry,western blotting was used to test the expression of Hsp75 and activating caspase-3 protein.RESULTS Compared with Aβ_(1-42) treatment group,resveratrol pretreament group significantly increased the C17. 2 cells survival rate( P>0. 05) and apoptosis rate( P>0. 05) in a dose-dependent manner. Similarly,resveratrol significantly ameliorate the expression level of Hsp75 protein and reduced the level of activated caspase-3 protein in C17.2 cells in Aβ_(1-42) treatment group. However,there was not significant effect of resveratrol on the viability of C17. 2 cells( P > 0. 05). CONCLUSION Resveratrol has an obviously protective effect on Aβ_(1-42) induced damage of C17. 2 cells. The possible mechanism was that resveratrol up-regulated the expression of Hsp75 protein in C17.2 cells and inhibited the caspase-3 protein-associated apoptotic pathway.

关键词(KeyWords): 白藜芦醇;阿尔茨海默病;热休克蛋白75;神经干细胞
resveratrol;Alzheimer's disease;heat shock protein-75;nueral stem cell

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作者(Author): 朱江华;范焕琼;
Zhu Jiang Hua;Fan Huan Qiong;Foshan Women and Children Hospital;Pharmacy department,The 5th People's Hospital of Foshan City;

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